《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (07): 418-420.

• 基础研究 • 上一篇    下一篇

降纤酶对大鼠局灶性脑缺血再灌流损伤的影响

武小玲;尹岭;黄如训   

  1. 降纤酶实验研究课题协作组 1.解放军总医院神经信息中心、神经病学研究所;2.中山大学医学院附属一院神经内科;3.白求恩医科大学神经科;4.复旦大学附属华山医院
  • 收稿日期:2002-05-23 出版日期:2002-07-25 发布日期:2002-07-25

Experimental study on the therapeutic effectiveness of defibrase in rat focal cerebral ischemia reperfusion model

WU Xiao-ling,YIN Ling,HUANG Ru-xun   

  1. Center of Neuroinformatics,General Hospital of PLA
  • Received:2002-05-23 Published:2002-07-25 Online:2002-07-25

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摘要： 目的探讨降纤酶对局灶性脑缺血再灌流损伤的保护作用。方法用线栓法制成大鼠大脑中动脉闭塞/再灌流模型,182只大鼠分为:正常对照组;假手术组;缺血3h再灌流(3h、6h、24h、72h),缺血6h再灌流(3h、6h、24h)组,缺血再灌注组分为治疗组:再灌流前30min尾静脉注射降纤酶(8U/Kg),对照组:静脉注射等容量生理盐水。行大鼠神经功能评分;脑组织TTC染色,计算梗塞灶体积;HE染色观察脑组织病理变化。结果缺血3h再灌注大鼠降纤酶治疗组各时间点神经功能评分均较对照组低(P<0.05),梗死灶体积较对照组小(P<0.05)。缺血6h再灌注组降纤酶治疗组的神经功能评分和梗死灶体积与对照组比较无显著性差异(P>0.05)。降纤酶组未见脑内出血加重的倾向。结论降纤酶对大鼠局灶脑缺血3h再灌流引起神经功能障碍和脑组织损伤有保护作用。

关键词: 脑缺血再灌流, 降纤酶, 梗死灶体积, 大鼠

Abstract: ObjectiveTo investigate the therapeutic effectiveness of defibrase in treating penumbra and reperfusion.MethodsIntraluminal suture method was used to develope reversible middle cerebral artery occlusion(MCAO). Rats were subjected to MCAO 3 hours followed by reperfusion for 3, 6, 24, 72 hours, and to MCAO 6 hours followed by reperfusion for 3, 6, 24 hours. The treatment groups rats were injected intravenously defibrase at 0.5 hour before reperfusion. Meanwhile, the control group received normal saline. Clinically Neurological Deficits Scale were evaluated every day. Infarction volume was measured by using 2,3,5-triphenyltetrazolium chloride staining. Pathologic change were examined microscopically in HE stained sections.ResultsThere were significant difference at treatment groups of reperfusion 3 hours after MCAO.Infarction volume and Clinically Neurological Deficits Scale was significant reduced as control group (P<0.05). There were no significant differences at treatment groups of reperfusion 6 hours after MCAO (P>0.05). Cerebral hemorrhage wasn't increased in defibrase treatment group.ConclusionsDefibrase was effective on Clinically Neurological Deficits of rats in reperfusion 3 hours after MCAO.

Key words: cerebral ischemia reperfusion, defibrase, cerebral infarction volume, rat