《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (07): 421-422.

• 基础研究 • 上一篇    下一篇

脑溢安对谷氨酸致神经元损伤的保护作用

聂亚雄1;黎杏群1;黄亮群2;聂亚雄3;黄如训3   

  1. 1.中南大学附属湘雅医院中西医结合研究所 湖南长沙市 410008;2.中南大学中国医学遗传学国家重点实验室 湖南长沙市 410008;3.中山大学附属一院神经科 广东广州市 510080
  • 收稿日期:2002-05-16 出版日期:2002-07-25 发布日期:2002-07-25

Effect of serum obtained from rat treated orally with Traditional Chinese Medicine Nao Yi-An on MAPK signal transduction in injured cultured neurons

NIE Ya-xiong,LIXing-qun,HUANG Liang-qun,et al   

  1. Institute of Combined Chinese and Western Medicne,Xiangya Hospital,Changsha 410008,Hunan,China
  • Received:2002-05-16 Published:2002-07-25 Online:2002-07-25

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摘要： 目的探讨中医平肝熄风,凉血泻火治法的抗神经损伤机理。方法建立谷氨酸致体外培养的大鼠海马神经元兴奋毒性损伤模型,并用脑溢安含药血清、细胞外调节蛋白激酶(ERK)阻滞剂PD98059进行干预,分别检测各组神经元活化的ERK、c-jun氨基末端激酶(JNK)水平及上清液LDH活性。结果脑溢安含药血清能上调谷氨酸损伤后的大鼠海马神经元ERK水平,下调JNK水平,PD98059能阻断脑溢安对受损经元的保护作用。结论以平肝熄风、凉血泻火为主要治法的脑溢安含药血清对谷氨酸致培养大鼠海马神经元损伤后的保护作用与MAPK信号转导途径有关,脑溢安上调ERK表达,下调JNK表达,促进细胞生存。

关键词: 脑溢安, 血清药理学, 兴奋性氨基酸, 乳酸脱氢酶, 丝裂元活化的蛋白激酸, 培养神经元, 中医治法

Abstract: ObjectiveTo explore the effects of the serum of traditional Chinese medicine Nao Yi An on glutamate induced cell death in cultured hippocampal neurons of rat and the underlying mechanisms. MethodsHippocampal neurons were cultured. The excitatory amino acid induced toxicity on cultured neurons was investigated. The viability of injured neurons was determined with the measurement of Lactate dehydrogenase (LDH) activity. Mitogen activated protein kinase (MAPK) were determined by immunoprecipitation /kinase assays /western blot detection.ResultsThe serum of Nao Yi-An raised cell viability. The serum of Nao Yi-An upregulated the expression of extracellular regulated protein kinases(ERK) and downregulated the expression of c-Jun N terminal kinase/stress activited protein kinase(JNK) in cultured neurons. The serum of Nao Yi-An induced upregulation of ERK and its anti death action were prevented with the specific ERKs inhibitor PD98059. Conclusions Activation of ERK signaling together with inhibition of JNK signaling by Chinese medicine Nao Yi-An appears to be an important mechanism for its survival effects on cultured hippocampal neurons.

Key words: Nao Yi-An, serum pharmacology, excitatory amino acid, LDH, MAPK, cultured neurons, therapy of Traditional Chinese Medicine