《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (07): 426-427.

• 基础研究 • 上一篇    下一篇

中药复方水煎液对体外培养大鼠海马神经细胞缺氧损伤的保护作用

许健鹏1;徐基民1;赵蕾1;杨键2;董浩2;徐永琦2   

  1. 1.北京博爱医院 北京市 100077;2.中国康复研究中心医学研究所 北京市 100077
  • 收稿日期:2002-04-02 出版日期:2002-07-25 发布日期:2002-07-25

Protective effect of Chinese Medical Formula Decoction on anoxic damage in cultured hippocampal neuronal cells from newborn rats

XU Jian-peng,YANG Jian,XU Ji-min,et al   

  1. Beijing Bo’ai Hospital,Beijing 10077,China
  • Received:2002-04-02 Published:2002-07-25 Online:2002-07-25

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摘要： 目的探讨中药复方水煎液Ⅰ、Ⅱ、Ⅲ号对大鼠海马神经细胞缺氧损伤有无保护作用。方法16只Wistar大鼠,随机分为4组,第1组每天分两次灌喂生理盐水4ml,第2—4组分别每天分两次灌喂等量中药复方水煎液Ⅰ、Ⅱ、Ⅲ号,3天后取血,制备血清。另取新生48小时内的Wistar大鼠,在无菌条件下断头取脑,分离海马,制成细胞悬液体外培养。在培养第7天将培养皿随机分为5组,第1组为正常对照组,第2—5组分别加入灌喂生理盐水及中药复方Ⅰ、Ⅱ、Ⅲ号的大鼠血清,24小时后2—5组培养皿置缺氧环境1小时,之后再培养24小时,检测各培养皿细胞存活率及培养液中乳酸脱氢酶(LDH)活性和丙二醛(MDA)含量。结果灌喂复方Ⅰ号组和Ⅱ号组大鼠血清的培养液中LDH的活性和MDA的含量明显低于缺氧对照组,细胞存活率高于缺氧对照组,均有统计学意义(P<0.05—0.001)。结论中药复方Ⅰ号和Ⅱ号对体外培养大鼠海马神经元缺氧损伤具有保护作用。

关键词: 中药复方煎剂, 海马神经细胞, 缺氧损害

Abstract: ObjectiveTo study the protective effect of Chinese Medical Formula Decoction on anoxic damage in cultured hippocampal neuronal cells from newborn rats. MethodsThe sera with Chinese Medicine (SCM-1,2,3) were collected from rats fed on Chinese Medical Formula Decoction Ⅰ,Ⅱ,Ⅲ(CMFD-Ⅰ,Ⅱ,Ⅲ) for 3 days,while the sera of rats which were fed with normal saline was collected as control. Hippocampal neuronal cells were obtained from dissociated cerebrella of 2 day old Wister rats. The cells were maintained in 5% CO2 humidified atmosphere at 37℃. After 7 days, the cells were grown in culture media containing SCM-1,2,3 and normal saline for 24 hours and in anoxic atmosphere for 1 hour. After anoxia, the cells were cultured for 24 hours. Culture media were collected and Malonaldehyde (MDA) and Lactate Dehydrogenase (LDH) in culture media were detected. ResultsThe survival neuronal cell rate in SCM-1 and 2 groups were significantly higher than control group. LDH and MDA in culture media of SCM-1 and 2 groups were lowed than control group. Conclusions There is a protective effect of CMFD-1 and 2 on anoxic damage in cultured hippocampal neuronal cells from newborn rats.

Key words: Chinese Medical Formula Decoction, hippocampal neuronal cells, anoxic damage