《中国康复理论与实践》

《中国康复理论与实践》 ›› 2002, Vol. 8 ›› Issue (09): 529-530.

• 基础研究 • 上一篇    下一篇

联合用药在急性脑缺血再灌注损伤中保护作用的实验研究

朴春花; 纪树荣; 许健鹏   

  1. 北京博爱医院综合康复科 北京市 100077
  • 收稿日期:2002-04-22 出版日期:2002-09-25 发布日期:2002-09-25

Experimental study of the protective effect of combined medication on acute cerebral ischemic reperfusion injury

PIAO Chun-hua, JI Shu-rong, XU Jian-peng   

  1. Department of Rehabilitation,Beijing Charity Hospital,Beijing 100077,China
  • Received:2002-04-22 Published:2002-09-25 Online:2002-09-25

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被引次数

1

摘要： 目的筛选在急性脑缺血再灌注过程中对脑有较好保护作用的用药方法。方法采用动脉夹钳闭大白鼠双侧颈内动脉,夹闭3小时后开放动脉钳恢复血流,完成脑缺血再灌注动物模型。 40只1个月龄雌性Wistar大鼠,随机分成5组,每组8只。A组:缺血再灌注尼莫地平(0.2mg/kg)治疗组;B组:缺血再灌注甘露醇(0.5g/kg)治疗组;C组:缺血再灌注尼莫地平及甘露醇联合治疗组;D组(模型组):缺血再灌注无药物治疗组;E组(假手术组):除未进行动脉夹闭,其他手术过程同前几组,而且无药物治疗干预。于再灌注24小时后检测各组脑组织及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量及行脑组织病理检查进行比较。结果C组较A、B组脑组织中的SOD、MDA及脑组织病理检查比较结果均有显著差异(P<0.01)。结论尼莫地平与甘露醇联用在急性脑缺血再灌注实验中对脑组织有较好的协同保护作用。

关键词: 脑缺血再灌注, 尼莫地平, 甘露醇, 联合治疗, 相互作用

Abstract: ObjectiveTo study neural protective effect of combined medication with nimodipine and mannitol on injury of cerebral ischemia-reperfusion for screening the better medication method in acute cerebral ischemia-reperfusion. MethodsA model of cerebral ischemia-reperfusion was performed by clipping bilateral common carotid artery of rats with vago- and releasing them 3 hours later. 40 Wistar female rats within 1 month were divided into 5 groups randomly with 8 rats each: model group (no use of medicine), nimodipine group(0.2mg/kg), mannitol group(0.5g/kg), nimodipine and mannitol group, sham-operated group (no use of medicine and no clipping process). The changes of SOD and MDA in brain tissue were measured 24 hours after cerebral ischmic reperfusion in all groups. At the same time pathologic study was performed to compare the different groups.ResultsThere were significant differences between nimodipine and mannitol group and other groups in changes of SOD, MDA and pathological changes(P<0.01). Conclusions Combined medication with nimodipine and mannitol is the better way to protect brain tissue from acute cerebral ischemia-reperfusion than other way in present experiment, by synergistic action.

Key words: ischemia-reperfusion, nimodipine, mannitol, combined medication, synergistic action