《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (05): 261-262.

• 专题研究 • 上一篇    下一篇

胆红素脑病仔兔黑质-纹状体系神经元计数及超微结构的研究

李晓捷1; 朴文花2; 李林1; 姜志梅1   

  1. 1.黑龙江省佳木斯大学康复医学院 154002;2.沈阳市儿童医院 110000
  • 收稿日期:2003-04-30 出版日期:2003-05-25 发布日期:2003-05-25

Study of ultrastructure and neuron quantitative determination in nigra-striatum of newborn rabbits with bilirubin encephalopathy

LI Xiao-jie,PIAO Wen-hua,LI Lin,et al   

  1. College of Rehabilitation Medicine, Jiamusi University, Heilongjiang 154002,China
  • Received:2003-04-30 Published:2003-05-25 Online:2003-05-25

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被引次数

14

摘要： 目的探讨胆红素对黑质-纹状体系神经元的毒性作用及损伤程度。方法 30只纯系日产大耳白仔兔随机分为3组:对照组(C组,6只)腹腔注射生理盐水1ml,注射后6小时灌杀;模型1组 (N1组,12只)腹腔注射胆红素100mg/kg,分别于给药后6小时(N1a组,6只)、16小时(N1b组,6只)灌杀;模型2组(N2组,12只)腹腔注射胆红素200mg/kg,分别于给药后6小时(N2a组,6只)、16小时(N2b组,6只)灌杀。用图像分析仪进行细胞计数,电镜分析超微结构变化。结果N1b组黑质神经元数少于C组、N1a组(P<0.05),N2b组黑质神经元数明显少于N1b、N2a组(P<0.05)的黑质-纹状体系神经元数量减少,神经元超微结构有不同程度的改变。结论胆红素能减少黑质-纹状体系神经元数量,且剂量越大、时间越长,减少越明显;胆红素脑病时黑质、纹状体系神经元超微结构改变明显,且剂量越大、时间越长损伤越重;黑质、纹状体系的形态、计量学变化呈现一致性。

关键词: 胆红素脑病, 黑质-纹状体系, 兔

Abstract: ObjectiveTo explore the noxious effect and the extent of damage in nigra-striatum neuron of bilirubin.Methods30 newborn rabbits were divided into 3 groups randomly:control group(group C,12 rabbits) which were injected normal salt intraperitoneally,model group 1(group N1,12 rabbits) which were injected bilirubin 100mg/kg intraperitoneally, model group 2(group N2,12 rabbits) which were injected bilirubin 200mg/kg intraperitoneally. All the rabbits in group C and 6 rabbits in group N1(group N1a) and group 2(group N2a) were killed 6 hours after injection.Other 6 rabbits in group N1(group N1b) and group 2(group N2b) were killed 16 hours after injection. The quantity of neuron in nigra-striatum were determined,and the changes of ultrastructure were observed by electron microscope.ResultsThe neuron in nigra-striatum in group N2b were decreased compared with group C and group N1a(P<0.05),as well as with group N1b and N2a. The ultrastructure of the neuron was changed.ConclusionsThe changes in utrastructure and the quantity of nigra-striatum neuron were associated with the concentration and time of exposure under bilirubin. It is consisted with the changes of ultrastructrue and quantity in nigra-striatum.

Key words: bilirubin encephalopathy, nigra-striatum, rabbit