《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (05): 290-292.

• 基础研究 • 上一篇    下一篇

大肠癌细胞DNA倍体及增殖活性与临床病理学的关系

高飞1; 国永生2; 王宇2   

  1. 1.北京博爱医院外科 北京市 100077;2.北京友谊医院普外科 100051
  • 收稿日期:2003-02-22 出版日期:2003-05-25 发布日期:2003-05-25

Relation of DNA ploidy and proliferative activity with the clinical and pathologic features in colorectal cancer

GAO Fei,GUO Yong-sheng, WANG Yu   

  1. Department of Surgery,Beijing CharityHospital,Beijing 100077,China
  • Received:2003-02-22 Published:2003-05-25 Online:2003-05-25

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摘要： 目的研究癌细胞的DNA异倍体及增殖活性与大肠癌的临床病理因素的关系。方法应用流式细胞仪对119例新鲜大肠癌组织细胞的DNA倍体及细胞周期各相中的分布进行检测,与大肠癌的血清癌胚抗原、癌肿大小、形态、淋巴结转移、Dukes’分期、组织学类型及组织分化程度等病理因素进行前瞻性研究比较。结果血清癌胚抗原与癌细胞的DNA异倍体及增殖活性均无关。溃疡型中的DNA异倍体出现率为56.5%,显著高于隆起型(14.7%)(P<0.01)。中低分化组中的异倍体出现率为64.7%,显著高于高分化组(36.5%)(P<0.01)。癌肿大小、淋巴结转移、Dukes’分期及癌组织学类型均与DNA异倍体无关。大肠癌上述各临床病理因素均与癌细胞增殖活性无关。结论癌细胞DNA异倍体能反映大肠癌的恶性程度,但癌细胞增殖活性(SPF、PI)与各临床病理因素无关。血清癌胚抗原与癌细胞DNA倍体及增殖活性无关。

关键词: 大肠癌, DNA异倍体, 增殖活性

Abstract: ObjectiveTo study the relationship between DNA aneuploidy and proliferative activity and the clinical and pathologic features.MethodsDNA ploidy and cell cycle analysis were analyzed in 119 colorectal fresh cancer apecimens using flow Cytometry and prospectively compared with the CEA in serum,tumor size,tumor morphology,lymph node metastases,Dukes' Classfication,histologic type and grade in colorectal cancer.ResultsThere was no relation between serum CEA and DNA aneuploidy and proliferative activity. Aneuploidy was detected at 56.5% in ulcerating carcinoma, which was significantly higher than 14.7% in bulge carcinoma (P<0.01). Aneuploidy was detected at 64.7% in middle and lower grade carcinoma, which was significantly higher than 36.5% of high grade carcinoma (P<0.01). No significant differences in aneuploidy were observed with respect to tumor size, lymph node metastases, Dukes' classfication,tumor histologic type.ConclusionsDNA aneuploidy of cancer cell can express the degree of malignancy of colorectal cancer. But proliferative activity does not relate to all the clinical and pathologic features. CEA in serum does not relate to DNA aneuploidy and proliferative activity.

Key words: colorectal cancer, DNA aneuploidy, proliferative activity