《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (05): 293-295.

• 基础研究 • 上一篇    下一篇

双龙丸对大鼠实验性心肌梗死血管新生的影响与分子学机制

杨祖福1,2; 胡婉英2,3; 秦志强4; 张明彩4; 付继航4   

  1. 1.北京博爱医院内科 北京市 100077;2.上海中医药大学 上海市 200032;3.上海中医药大学附属曙光医院心内科 上海市 200032;4.新乡医学院第一附院病理科 河南卫辉市 453100
  • 收稿日期:2002-11-20 出版日期:2003-05-25 发布日期:2003-05-25

Angiogenesic effects and molecular mechanisms of Shuanglong Pill on experimental myocardial infarction rats

YANG Zu-fu,HU Wan-ying,QIN Zhi-qiang,et al   

  1. Department of Internal Medicine,Beijing Charity Hospital, Beijing 100077, China
  • Received:2002-11-20 Published:2003-05-25 Online:2003-05-25

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摘要： 目的探讨双龙丸对缺血心肌血管新生的影响和分子学机制。方法 63只Wistar大鼠参照Drexler法结扎冠状动脉左前降支,制成急性心肌梗死(AMI)模型。随机分为双龙丸大剂量组 (6.72g/kg)、双龙丸小剂量组(3.36g/kg)、AMI对照组(生理盐水灌胃),另取11只大鼠为正常对照组(生理盐水灌胃),各组治疗观察半数至2W、半数至4W结束。应用免疫组化两步法检测AMI大鼠缺血心肌新生血管数量、免疫组化SP法检测缺血心肌血管内皮生长因子(VEGF)和碱性成纤维细胞生长因子(bFGF)的表达、逆转录多聚酶链反应(RT-PCR)法检测缺血心肌VEGFmRNA和bFGFmRNA的表达。结果双龙丸大、小剂量组各期的缺血心肌新生血管数均比AMI对照组增多,且第4W比第2W更显著;第2W时,大剂量组VEGF较AMI对照组、bFGF较小剂量组和AMI对照组均增高,大剂量组4W时VEGF较2W降低;大、小剂量组各期VEGFmRNA的表达均比AMI对照组增高,其第4W均较第2W为低;而bFGFmRNA的表达则仅见大剂量组第2W时高于其他组;以上各组比较均具有显著性差异(P<0.05—0.01)。结论双龙丸能够促进缺血心肌血管新生;大剂量应用对VEGF、bFGF及其mRNA均有明显的上调作用。

关键词: 双龙丸, 心肌梗死, 血管新生, 血管内皮生长因子, 碱性成纤维细胞生长因子, 大鼠

Abstract: ObjectiveTo study the effects and molecular mechanisms of Shuanglong Pill on angiogenesis of ischemic myocardium. Methods63 Wistar rats were ligated left anterior descending branch to be made into models of acute myocardial infarction(AMI).All rats were randomly divided into large-dosage group of Shuanglong Pill(LDG),small-dosage group of Shuanglong Pill(SDG),The control of acute MI(MIC).Other 11 rats were set as normal control(NC).Rats in every group had been treated half for 2 weeks and half for 4 weeks. New vessels' quantity in ischemic myocardium of acute MI rats were detected by immunohistochemistry double steps assay;the expressions of vessel endothelial growth factor(VEGF) and basic fibroblast growth factor(bFGF) on ischemic myocardium were detected by immunohistochemistry SP assay;the expressions of VEGF mRNA and bFGF mRNA on ischemic myocardium were detected by reverse transcription polymerase chain reaction(RT-PCR) assay.ResultsThere were more new capillaries on ischemic myocardium of LDG, SDG compared with that in MIC at every stage,moreover,there was significantly increase at the end of 4 weeks compared with that at 2 weeks.At the end of 2 weeks, the expression of VEGF in LDG were significantly elevated compared with that in MIC; and the expression of bFGF in LDG were significantly elevated compared with that in SDG and MIC.Whereas the expression of VEGF in LDG were significantly decreased at the end of 4 weeks compared with 2 weeks. There were significant differences of above comparison (P<0.05-0.01).At every stage the expression of VEGF mRNA in LDG, SDG were significantly elevated compared with that in MIC; but its expression was significantly decreased at the end of 4 weeks compared with that 2 weeks. The expression of bFGF mRNA was significantly elevated in LDC at the end of 2 weeks compared with that in other groups.ConclusionsShuanglong Pill could promote angiogenesis in ischemic myocardium,application of large dose of Shuanglong Pill could significantly upregulate the expression of VEGF, bFGF, VEGF mRNA and bFGF mRNA.

Key words: Shuanglong Pill, myocardial infarction, angiogenesis, vessel endothelial growth factor, basic fibroblast growth factor, rat