《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (07): 390-392.

• 专题研究 • 上一篇    下一篇

β-淀粉样肽对小鼠海马神经发生的影响及中药的干预作用

张孟仁1; 左萍萍2; 李学坤2; 孔令娜2; 郭赛珊1   

  1. 1.北京协和医院中医科 北京市 100730;2.中国协和医科大学基础医学院药理系 北京市 100005
  • 收稿日期:2003-06-30 出版日期:2003-07-25 发布日期:2003-07-25

Effects of β-Amyloid peptide on neurogenesis in brain hippocampus of mice and interfering effect of traditional Chinese medicine

ZHANG Men-ren,ZUO Ping-ping,LI Xue-kun,et al   

  1. Institute of Basic Medical Sciences,Beijing Union Hospital,Beijing 100005,China
  • Received:2003-06-30 Published:2003-07-25 Online:2003-07-25

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摘要： 目的观察Aβ25-35对痴呆小鼠模型海马神经发生的影响及中药“脑复聪”的干预作用。方法脑室内注射Aβ25-35造成痴呆模型小鼠。用Morris水迷宫测空间学习记忆功能;同位素配基测受体结合活性;BrdU标记的免疫组化检测神经发生。结果模型小鼠的学习记忆功能降低(P<0.05);海马NMDA受体结合活性显著升高而胆碱能M受体结合活性显著降低(P<0.001);海马齿状回BrdU阳性细胞减少(P<0.05)。经“脑复聪”治疗后上述变化均得到改善(P<0.05,P<0.001,P<0.05)。结论Aβ25-35的神经毒机理包括对神经发生的抑制作用;提高神经发生率可能是中药治疗神经退行性疾病的重要机理之一。

关键词: β-淀粉样肽, 神经发生, 海马, 中药

Abstract: ObjectiveTo observe the changes of neurogenesis in the hippocampus of Alzheimer's model mice and the effect of traditional Chinese medicine Nao Fu Cong. MethodsAlzheimer's type dementia of mice was induced by Aβ25-35 icv.Space learning and memorial ability were tested with Morris Water Maze.The activity of NMDA and M receptor were measured with radio-ligand of MK-801 and QNB.Neurogenesis was observed with the BrdU immunohitochemistry.ResultsSpace learning and memorial ability significantly decreased(P<0.05),MK-801 binding increased and QNB binding decreased (P<0.001),BrdU positive cells decreased in hippocampus(P<0.05).After given drugs for 2 weeks,the mentioned changes were improved significantly(P<0.05,P<0.001,P<0.05).ConclusionsThe toxic effect of Aβ25-35 was involved with the inhibitory action of neurogenesis.Promoting the neurogenesis may be one of the mechanism of traditional Chinese medicine to treat the neurodegenerative diseases.

Key words: Amyloid peptide, neurogenesis, hippocampus, traditional Chinese medicine