《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (07): 394-396.

• 专题研究 • 上一篇    下一篇

大鼠胎鼠中脑神经干细胞的分离培养

李学坤; 郭安臣; 左萍萍   

  1. 中国医学科学院、中国协和医科大学基础医学院药理学系 北京市 100005
  • 收稿日期:2003-06-30 出版日期:2003-07-25 发布日期:2003-07-25

Isolation and culture of neural stem cells in ventral midbrain of a rat embryo

LI Xue-kun,GUO An-chen,ZUO Ping-ping   

  1. Department of Pharmacology,PUMC&CAMS,Beijing 100005,China
  • Received:2003-06-30 Published:2003-07-25 Online:2003-07-25

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被引次数

3

摘要： 目的探讨神经干细胞分离、培养及鉴定的方法,并了解其生物学特性。方法分离大鼠胎鼠腹侧中脑组织,加入丝裂原bFGF进行克隆密度细胞培养。用免疫细胞化学方法进行神经干细胞及其分化细胞的鉴定。结果培养的神经干细胞具备神经干细胞的基本特性,以对称和不对称分裂方式增殖形成神经球,并分化为神经元、神经胶质细胞、少突胶质细胞。结论成功建立了神经干细胞的分离培养方法。

关键词: 大鼠胎鼠中脑, 神经干细胞, 分离, 培养

Abstract: ObjectiveTo establish a method of isolation and culture of neural stem cells(NSCs). MethodsTissues of ventral midbrain were isolated from a rat embryo,and the NSCs were cultured stimulated with basic fibroblast growth factor(bFGF)in vitro.The cells were identified by immunocytochemistry.ResultsNSCs proliferated into neurosphere in symmetric and non-symmetric cleavage ways,and differentiated into neuron, astroglia and oligodendrosyte. ConclusionsThe method has been established to isolate and culture the neural stem cells.

Key words: ventral midbrain of rat embryo, neural stem cell, isolation, culture