《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (07): 410-412.

• 基础研究 • 上一篇    下一篇

中药SSY-B2促进中枢神经系统损伤后轴突再生的分子机制

陆华宝1.2; 李林1; 安文林1; 张丽1; 叶翠飞1   

  1. 1.首都医科大学附属宣武医院 北京市 100035;2.北京博爱医院 北京市 100077
  • 收稿日期:2003-03-17 出版日期:2003-07-25 发布日期:2003-07-25

Effect of SSY-B2 on regeneration of central nervous system of rats with bilateral fornix/fimbria transaction

LU Hua-bao,LI Lin,AN Wen-lin,et al   

  1. Beijing Xuanwu Hospital,Beijing 100035,China
  • Received:2003-03-17 Published:2003-07-25 Online:2003-07-25

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被引次数

63

摘要： 目的观察中药SSY-B2对中枢神经系统神经再生的促进作用。方法成年雄性SD大鼠随即分为6组:假手术组、模型组、阳性对照药脑复康治疗组(0.56g纯药/kg)、SSY-B2小剂量治疗组(1.5g生药/kg)、SSY-B2中剂量治疗组(3g生药/kg)、SSY-B2大剂量治疗组(6g生药/kg)。大鼠行双侧隔海马穹窿伞离断术。术后给予SSY-B2等试验用药6周。在行为学测试结束后,采用免疫组织化学方法,在大脑有关区域测定生长相关蛋白-43(GAP-43 )、硫酸软骨素蛋白多糖(CSPG)。结果伤口周围GAP-43的阳性细胞数假手术组与模型组相比无显著性差异(P>0.05),SSY-B2小剂量能显著促进损伤后GAP-43的表达(与模型组相比:P<0.001)。损伤后顶叶皮层有CSPG表达,假手术组和模型组密度分别为(56.43±59.6)、(116.36±10.561),SSY-B2小、中剂量能抑制损伤后CSPG的表达(和模型组相比分别为P<0.05,P<0.01)。结论SSY-B2小剂量和脑复康能显著促进损伤后GAP-43的表达,抑制CSPG表达,起到促进中枢神经系统(CNS)神经再生的作用,而轴突再生可能在某种程度上与损伤后的结构和功能修复有关。

关键词: 中药, 中枢神经系统, 再生, 生长相关蛋白-43, 硫酸软骨素蛋白多糖

Abstract: ObjectiveTo study the effect of SSY-B2 on regeneration of central nervous system of rats with bilateral fornix/fimbria transaction.MethodsMale adult SD rats were divided randomly into 6 groups as sham group,model group, positive control agent piracetam group, SSY-B2 low dosage(1.5g crude drug/kg) group, medium dosage(3g crude drug/kg) group and high dosage(6g crude drug/kg) group.The bilateral fornix/fimbria transection in the rats were carried out. After operation, drugs were fed introgastrically to each group respectively for 6 weeks. The immunoreactive products of growth-associated protein-43(GAP-43 )and chondroitin sulfate proteoglycans(CSPG) in defined areas were measured using immunohistochemical methods. ResultsThere was no difference in number of cells expressing GAP-43 between the model group and sham group (P>0.05),but that in low dosage group increased compared with the model group (P<0.001). The CSPG in parietal lobes after lesion expressed,which was in sham group and model group(sham group and model group respectively, 56.43±59.6,116.36±10.561), and SSY-B2 in low and medium dosage inhibited its expression compared with the model group (P<0.05,P<0.01). Conclusions SSY-B2 can enhance the expression of GAP-43 and inhibit the expression or deposition of CSPG, promote axonal regeneration in the CNS, and thus structural repair and functional restoration in certain degree.

Key words: traditional Chinese medicine, central nervous system, regeneration, growth-associated protein-43, chondroitin sulfate proteoglycans