《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (07): 414-415.

• 基础研究 • 上一篇    下一篇

bFGF在大鼠脑弥漫性轴索损伤中的脑保护作用

李红旗1; 张玉芝2; 高荣丰1; 张战波1; 程钢1   

  1. 1.武警河北总队医院神经外科 石家庄 050081;2.白求恩国际和平医院 石家庄 050082
  • 收稿日期:2002-12-31 出版日期:2003-07-25 发布日期:2003-07-25

Neuroprotective effect of bFGF on cerebral diffuse axonal injuries of rats

LIHong-qi,ZHANG Yu-zhi,GAO Rong-feng,et al   

  1. Department of Neurosurgery,Hebei Provincial Corps Hospital,Chinese People‘s Armed Police Forces,Shijiazhuang 050081,Hebei,China
  • Received:2002-12-31 Published:2003-07-25 Online:2003-07-25

PDF (PC)

摘要： 目的观察碱性成纤维细胞生长因子(bFGF)在大鼠脑弥漫性轴索损伤(DAI)中的保护作用。方法选择健康雄性SD大鼠40只,随机分为正常对照组(5只)、DAI损伤组(25只)和bFGF组(5只)、DAI生理盐水组(5只),DAI损伤组大鼠按伤后存活时间又分为第6h、12h、24h、72h及7d组。用免疫组织化学方法检测大鼠脑皮层bFGF的表达,并分析其表达水平变化特点。bFGF治疗组处理同DAI损伤组,在脑损伤前2h向右侧脑室穿刺注射bFGF 10μl。结果DAI后6h出现bFGF表达,12h表达增加,72h达高峰,7d后仍维持较高水平,DAI损伤组72h组与其他各时限比较有高度显著性差异(P<0.01)。HE染色结果显示,DAI损伤组脑皮层神经元数量明显减少,与DAI生理盐水组相比,各时间点bFGF治疗组损伤的神经元数量减少(P<0.05)。结论bFGF在大鼠脑弥漫性轴索损伤中有明显的脑保护作用。

关键词: 弥漫性轴索损伤, 碱性成纤维细胞生长因子, 免疫组织化学

Abstract: ObjectiveTo observe the neuroprotective effect of basic fibroblast growth factor(bFGF)on cerebral diffuse axonal injuries (DAI)of rats.Methods40 male SD rats were randomly divided into control group, DAI injury group, bFGF group and DAI saline group. According to the survival time of rats, DAI injury group were divided into five groups, the 6h, 12h, 24h, 72h and 7th day group, The changes of bFGF expression in cerebral cortex were detected by immunohistochemical method from 6h to 7d after DAI. Two hours before DAI, bFGF 10μl was injected into right ventricle in bFGF group. ResultsThe bFGF expression appeared at 6h after DAI, increased at 12h, reached the highest level at 72h, and kept in a high level at 7d.There were obvious differences between 72h group and other groups in DAI injury group (P<0.01),cerebral cortex neurons were obviously decreased by HE staining. In each time group,injured neurons were decreased in bFGF group combined with DAI injury group(P<0.05). ConclusionsbFGF has obvious neuroprotective effect on cerebral diffuse axonal injuries of rats.

Key words: diffuse axonal injuries(DAI), basic fibroblast growth factor (bFGF), immunohistochemistry