《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (10): 598-599.

• 基础研究 • 上一篇    下一篇

脑复聪对PC12细胞拟缺血损伤保护作用的血清学研究

张孟仁1; 徐惠媛1; 郭赛珊1; 左萍萍2   

  1. 1.北京协和医院中医科 北京市 100730;2.中国协和医科大学基础医学院药理系 北京市 100005
  • 收稿日期:2003-08-11 出版日期:2003-10-25 发布日期:2003-10-25

Studies on the protective effect of drug serum taken from rats administered with Naofucong in PC12 cell ischemic model

ZHANG Meng-ren, XUHui-yuan, GUO Sai-shan, et al   

  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China
  • Received:2003-08-11 Published:2003-10-25 Online:2003-10-25

PDF (PC)

被引次数

29

摘要： 目的观察中药复方脑复聪颗粒对H2O2 损伤的大鼠肾上腺髓质嗜铬瘤分化细胞株(PC12细胞)的影响。方法选用SD雄性大鼠,分别灌胃脑复聪和蒸馏水后制备血清。培养的PC12细胞加入不同血清孵育后,再加入不同浓度的超氧化合物H2O2造成拟缺血模型,采用MTT法观察细胞生存率。结果对受不同剂量H2O2损伤的PC12细胞,脑复聪含药血清可明显提高其生存率(P<0.05—0.01);加入脑复聪血清后的神经细胞可明显抵抗H2O2所致的损伤,细胞形态基本恢复正常。结论脑复聪颗粒对H2O2损伤的PC12细胞有明显保护作用。

关键词: 中药, 脑复聪, 药物血清, PC12细胞, 缺血模型

Abstract: ObjectiveTo examine effects of traditional Chinese herbs Naofucong grain on PC12 cells injured by H2O2 in vitro MethodsRats random divided into 2 groups: Naofucong grain treating group (administered with Naofucong grain) and control group (administered with distilled water) After 3 days administration, serums were obtained and added to cultured PC12 cells which were under different concentrations of H2O2 Morphological examinations in PC12 cells were detected by MTT measurement ResultsMTT measurement showed that Naofucong grain administered rats serums significantly increased the survival number of PC12 cells in different concentrations of H2O2.Conclusion Naofucong grain may have obvious protective effects on PC12 cells injured by H2O2.

Key words: traditional Chinese herbs, Naofucong, drug serum, PC12 cells, ischemic model