《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (11): 641-642.

• 专题研究 •    下一篇

二苯乙烯苷对脑缺血小鼠脑组织含水量及自由基代谢的影响

刘治军1,2; 李林1; 叶翠飞1; 艾厚喜1   

  1. 1.首都医科大学宣武医院药理研究室,北京脑老化重点实验室 北京市 100053;2.卫生部北京医院临床药理基地 北京市 100730
  • 收稿日期:2003-09-17 出版日期:2003-11-25 发布日期:2003-11-25

Effects of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside on water content and the metabolism of free radicals in brain of ischemia mice

LIU Zhi-jun, LI Lin, YE Cui-fei, et al   

  1. Department of Pharmacology, Xuanwu Hospital of Capital Universityof Medical Science, Beijing Key Laboratory for Brain Aging, Beijing 100053,China
  • Received:2003-09-17 Published:2003-11-25 Online:2003-11-25

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摘要： 目的探讨2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(简称二苯乙烯苷)对缺血再灌注小鼠脑保护作用的机理。方法夹闭小鼠双侧颈总动脉,撤去动脉夹再灌注后迅速断头取脑。采用干-湿重法测定脑组织含水量;应用硫代巴比妥酸法测定脑组织中丙二醛(MDA)的含量,采用亚硝酸盐法测定过氧化物歧化酶(SOD)活性。结果模型组脑组织含水量明显高于假手术组。二苯乙烯苷灌胃给药各剂量组可降低模型小鼠的脑组织含水量;模型组脑组织SOD活性比假手术组降低,MDA的含量升高;与模型组相比,二苯乙烯苷治疗组SOD活性升高,MDA含量降低。结论二苯乙烯苷可以减轻脑组织水肿,增加脑组织抗氧化能力,可能具有脑保护作用。

关键词: 二苯乙烯苷, 脑缺血, 脑水肿, 氧自由基, 小鼠

Abstract: ObjectiveTo explore the mechanism of protection of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside(TSG) on brain.MethodsThe bilateral carotid arteries of mice were occluded and then reperfused. The mice were sacrificed to get the brain tissue. Brain water content(BWC) was assayed by dry-wet method; malondialdehyde (MDA) content was determined by thiobarbiturate method and the activity of superoxide dismutase(SOD) was estimated by nitrite salt assay.ResultsBWC of model group was significantly higher than that of sham-operation group, while the TSG treatment reduced the BWC remarkably in mice with ischemia-reperfusion. SOD activity of model group was significantly lower than that of sham-operation group, while MDA content increased remarkably. Comparing with the model group, the SOD activity of mice treated with TSG was higher and the MDA content was lower.ConclusionTSG may reduce brain edema, improve the anti-oxidative ability of the brain tissue, and therefore, have protective effects on the brain.

Key words: tetrahydroxystilbene-2-O-β-D-glucoside(TSG), cerebral ischemia, brain edema, free radicals, mouse