《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (11): 643-645.

• 专题研究 • 上一篇    下一篇

二苯乙烯苷对痴呆小鼠学习记忆及自由基代谢的影响

楚晋; 叶翠飞; 李林   

  1. 首都医科大学宣武医院药理研究室,北京脑老化重点实验室 北京市 100053
  • 收稿日期:2003-08-18 出版日期:2003-11-25 发布日期:2003-11-25

Effects of stilbene-glycoside on learning and memory function and free radicals metabolism in dementia model mice

CHU Jin,YE Cui-fei, LI Lin   

  1. Department of Pharmacology, Xuanwu Hospital of Capital University of Medical Science, Beijing Key Laboratory for Brain Aging, Beijing 100053,China
  • Received:2003-08-18 Published:2003-11-25 Online:2003-11-25

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摘要： 目的观察二苯乙烯苷(TSG)对β淀粉样肽致痴呆(AD)模型小鼠学习记忆及自由基代谢的影响。方法向小鼠右侧脑室定向注射Aβ1-40建立痴呆模型,并以TSG灌胃预防给药,各组小鼠均作Morris水迷宫及避暗测试后进行丙二醛(MDA)含量、单胺氧化酶(MAO-B)活性、总抗氧化能力(T-AOC)的测定。结果与对照组相比,模型组小鼠学习记忆能力降低(P<0.05),大脑皮层MDA含量和MAO-B活性显著升高(P<0.01);TSG能明显提高模型小鼠学习记忆能力(P<0.01),明显降低皮层MDA含量(P<0.01)和降低MAO-B活性(P<0.05),并提高皮层总抗氧化能力(T-AOC)(P<0.01)。结论TSG能明显提高学习记忆能力,减轻大脑脂质过氧化水平,对AD的防治具有良好的应用前景。

关键词: 二苯乙烯苷(TSG), β-淀粉样肽, 痴呆, 学习记忆, 阿尔茨海默病

Abstract: ObjectiveTo observe the effect of 2,3,5,4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG) on learning and memory ability and free radicals metabolism in dementia model mice induced by β-amyloid (Aβ).MethodsAβ1-40 was given to the right lateral ventricle in the model group, and the TSG had been administered to the therapy group for 8 weeks by gastrogavage.All the mice of different groups were tested with Morris water maze and step-through test. Then the mice were killed and biochemical assays of neurol MDA,MAO-B,T-AOC were performed.ResultsThe model mice showed worse ability in learning and memory compared with control mice. The MDA cotent, MAO-B activity in the cortical increased in model mice compared with normal control; TSG reduced the MDA content, MAO-B activity,and increased T-AOC activity.ConclusionTSG can improve the learning and memory ability of model mice, decrease peroxidation level of brain, and increase antioxidation ability of brain, which suggest that TSG may have a promising application in treatment of dementia disease such as AD.

Key words: 4'-tetrahydroxy stilbene-2-O-β-D-glycoside, β-amyloid, dementia, learning and memory, Alzheimer's disease(AD)