《中国康复理论与实践》

《中国康复理论与实践》 ›› 2003, Vol. 9 ›› Issue (11): 648-649.

• 专题研究 • 上一篇    下一篇

中药SSY-B2对大鼠穹窿伞切断后脑小胶质细胞的影响

陆华宝1; 李林2; 安文林2; 张丽2; 叶翠飞2   

  1. 1.北京博爱医院 北京市 100077;2.首都医科大学宣武医院 北京市 100035
  • 收稿日期:2003-03-17 出版日期:2003-11-25 发布日期:2003-11-25

Effect of SSY-B2 on microglial cells in rats after bilateral fornix/fimbria transection

LU Hua-bao,LI Lin,AN Wen-lin,et al   

  1. Beijing Xuanwu Hospital,Beijing 100035,China
  • Received:2003-03-17 Published:2003-11-25 Online:2003-11-25

PDF (PC)

摘要： 目的观察SSY-B2对大鼠脑伤口周围小胶质细胞的影响。方法成年雄性SD大鼠随机分为6组:假手术组、模型组、阳性对照药脑复康治疗组(0.56g纯药/kg)、SSY-B2小剂量组(15g生药/kg)、SSY-B2中剂量组(3g生药/kg)、SSY-B2大剂量组(6g生药/kg)。造模前0.5—1小时灌胃给予实验用药。造模后持续用药6周,断头取脑,采用BS-IB4(IsolectinB4fromBandeiraeaSimPlifolia ,一种标记小胶质细胞的凝集素)免疫组织化学方法观察伤口周围小胶质细胞的增殖。 结果假手术组和模型组细胞数分别为(30.3±21.8)、(114.5±102.3),P<0.05;SSY-B2 3个剂量组细胞数分别为(249.7±149.4)、(252.0±191.7)、(244.2±154.9),与模型组相比,均P<0.05。结论SSY-B2使伤口周围小胶质细胞数量增多,有助于损伤后的神经修复和功能改善。

关键词: 中药, SSY-B2, 小胶质细胞, 大鼠

Abstract: ObjectiveTo observe the effect of SSY-B2 on microglial cells in rats after bilateral fornix/fimbria transection.MethodsMale adult SD rats were divided randomly into 6 groups as sham group,model group,positive control agent piracetam group, SSY-B2 low(1.5g crude drug/kg), medium(3g crude drug/kg)and high dosage (6g crude drug/kg)groups. Half to 1 hour before operation, water or drugs were fed introgastrically to each group respectively and continued for 6 weeks.The tissues of brain was gained and the immunoreactive products of BS-I B4 (Isolectin B4 from Bandeiraea Simplifolia, a marker for microglia) in the perilesion area was measured using immunohistochemical methods.ResultsThe number of microglia of the sham and model groups was (30.3±21.8) and (114.5±102.3) respectively, P<0.05. That of three different dosage of SSY-B2 groups was(249.7±149.4), (252.0±191.7)and (244.2±154.9), P<0.05 for each group compared with the model group.ConclusionMicroglia number in the perilesion area can be increased by SSY-B2, which may contribute to the nerve repair and functional improvement after injury.

Key words: traditional Chinese medicine, SSY-B2, microglia, rats