《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 11-12.

• 专题 • 上一篇    下一篇

离体培养神经干细胞的超微结构学研究

安沂华; 翟晶; 历俊华; 张绍东; 孙异临; 王忠诚   

  1. 北京市神经外科研究所 北京市 100050
  • 收稿日期:2003-12-21 出版日期:2004-01-25 发布日期:2004-01-25

Ultrastructure of neural stem cells of Wistar rats cultured in vitro

AN Yi-hua, ZHAI Jing, LIJun-hua, et al   

  1. Beijing Neurosurgical Institute, Beijing 100050,China
  • Received:2003-12-21 Published:2004-01-25 Online:2004-01-25

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被引次数

38

摘要： 目的研究离体培养条件下神经干细胞的超微结构。方法分离、培养和诱导分化来自孕17-19d Wistar大鼠胎鼠大脑皮层的神经干细胞,分别用扫描电镜和透射电镜观察神经干细胞分化前、后的超微结构。结果未分化的神经干细胞的大小、形态和细胞内结构基本相同。细胞都呈小球形,表面带有微绒毛,细胞以有丝分裂形式进行增殖。透射电镜显示细胞核、浆比例很高,核呈多形性、分叶状;胞浆稀少,无成熟的细胞器。分化后,从球体表面向四周伸出众多突起,胞体也从球形变成扁平形,细胞浆体积明显增多,胞浆内出现多种成熟的细胞器。细胞从具有单一的细胞外部形态和内部结构的干细胞向不同的神经元和神经胶质细胞转变。结论神经干细胞是保留原始细胞外部形态、内部结构和增殖特性的特殊细胞,在一定条件下可以被诱导分化成具有成熟细胞器的神经元和胶质细胞。

关键词: 神经干细胞, 超微结构, 分化, 细胞器, 大鼠

Abstract: ObjectiveTo detect the ultrastructure of neural stem cells (NSCs) cultured in vitro .MethodsNSCs separated from the cortex of 17—19 days Wistar rat fetus were cultured and induced to differentiate in vitro. Electron microscopes were used to visualize the ultrastructure of these cells before and after differentiation.ResultsNSCs had the similar cellular size, morphology and intracellular structures pre-differentiation. Cells were able to proliferate via mitosis. The nucleus/cytoplasm ratio was very high. The nucleus was poly-morphological. Cells had very little cytoplasm and no mature organelles. After differentiation, several processes protruded out from cellular surface. Cells became flat shape, the volume of cytoplasm increased dramatically and various kinds of mature organelles appeared in the cytoplasm. Cells differentiated into two kinds of cells,neural cells and glial cells,with quite different morphology and intracellular structure. ConclusionNSC is one kind of original cells which can be induced to differentiate into mature neural cells and glial cells.

Key words: neural stem cells, ultrastructure, differentiation, organelle, rats