《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 13-14.

• 专题 • 上一篇    下一篇

骨髓间充质干细胞体外诱导分化为神经样细胞的机制

褚倩; 王亚平; 傅新巧; 张苏明   

  1. 华中科技大学同济医学院附属同济医院神经科 湖北武汉市 430030
  • 收稿日期:2003-12-21 出版日期:2004-01-25 发布日期:2004-01-25

Mechanism of differentiation of mesenchymal stem cells into neuron-like cells in vitro

CHU Qian, WANG Ya-ping, FUXin-qiao, et al   

  1. Department of Neurology, Tongji Hospital, Tongji Medical School of Huazhong University of Science and Technology, Wuhan 430030, Hubei, China
  • Received:2003-12-21 Published:2004-01-25 Online:2004-01-25

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被引次数

28

摘要： 目的探索骨髓间充质干细胞(MSCs)在体外诱导分化为神经样细胞的机制。方法分离培养大鼠MSCs,用二甲基亚砜(DSMO)和丁羟茴醚(BHA)诱导分化,检测诱导分化前、预诱导24h、诱导分化后6h、24h和48h神经细胞和神经干细胞的特异性标记蛋白的表达。结果诱导分化后,大部分MSCs变成双极、多极和锥形,并相互交织成网络结构,巢蛋白(Nestin)在诱导分化前不表达,在诱导分化后6h达到最高,24h和48h逐渐降低。神经元特异性核蛋白(NeuN)在诱导分化前不表达,在诱导分化后6h出现表达,24h和48h表达增强。结论MSCs经体外诱导先分化为神经干细胞,然后分化为神经元样细胞。

关键词: 骨髓间充质干细胞, 分化, 神经元样细胞, 神经干细胞

Abstract: ObjectiveTo study the mechanism of differentiation of mesenchymal stem cells(MSCs) into neuron-like cells in vitro.MethodsMSCs of Wistar rats were separated and cultured, and then induced with DMSO and BHA in vitro. The specific marking proteins of neurons, glia and neural stem cells were detected before preinduction, at 24h after preinduction, at 6h, 24h, and 48h after neuronal induction.ResultsAfter the inducement, many MSCs turned into bipolar,multipolar and taper,and then intersected as network structure. Nestin was strong positive at 6h after neuronal induction, and decreased at 24h, 48h after the induction. NeuN was present at 6 h after neuronal induction, and increased at 24h, 48h after the induction.ConclusionMSCs can be induced into neural stem cells(NSCs) at first, and then differentiate into neuron-like cells in vitro.

Key words: mesenchymal stem cells(MSCs), differentiation, neuron-like cells, neural stem cells(NSCs)