《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 15-16.

• 专题 • 上一篇    下一篇

人骨髓基质干细胞体外诱导成脂过程形态学变化的观察

赵琳; 杨志明; 邓力; 解慧琪; 李秀群   

  1. 四川大学华西医院卫生部移植工程与移植免疫重点实验室组织工程研究室 四川成都市 610041
  • 收稿日期:2003-10-27 出版日期:2004-01-25 发布日期:2004-01-25

Observation of the morphological change during the adipogenesis differentiation of human bone marrow stromal cell

ZHAO Lin,YANG Zhi-ming, DENG Li, et al   

  1. Key Laboratory of Transplant Engineering and Immunology of Ministry of Public Health: Institute of Reparative and Reconstructive Surgery Laboratory of Tissue Engineering, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China
  • Received:2003-10-27 Published:2004-01-25 Online:2004-01-25

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摘要： 目的了解骨髓基质干细胞(MSC)诱导成脂分化过程的形态变化,为诱导分化阶段的识别提供部分实验依据。方法由健康成年女性骨髓组织分离而得的MSC体外培养、扩增后,使用成脂诱导复合培养液诱导6—30d,每日在OlymPus相差显微镜下观察细胞形态变化或油红-O染色。结果MSC在诱导分化前呈长梭形,随着分化的进程,逐渐趋于椭圆、类圆、直至圆形 ,胞体也逐渐变大。细胞在分化过程中特征性的形态学变化是细胞内脂滴的变化(相当于不成熟脂肪细胞和成熟脂肪细胞阶段),从无到有,从胞膜下脂肪小颗粒到脂滴、脂泡,经历脂滴的增多和融合过程,直至整个细胞充满大脂泡,细胞分化进入终末阶段。结论MSC成脂诱导的过程中,分化进入不成熟及成熟脂肪细胞阶段因有特征性的脂滴变化而易识别,但从MSC分化到前脂肪细胞阶段并无明显的形态学特征,可能需通过特异性表达产物来鉴定。

关键词: 骨髓基质干细胞, 诱导, 分化, 成脂, 形态学

Abstract: ObjectiveTo study the variant differentiated phase of the human bone marrow stromal cell (MSC) during induced adipogenesis course by morphological observation.MethodsAfter proliferated in vitro, MSCs isolated from bone marrow of the female adults volunteers were cultured with the adipogenetic inducers for 6—30 days. Morphological changes of the cells were observed everyday under Olympus contrast phase microscopy, and some specimens were stained with Oil-red-O.ResultsMSC showed long spindle shape before inducing, and then changed gradually to oval or round shape and the figure enlarged simultaneously. There were specifically morphological changes with lipid droplet emergence under plasmalemma and confluence gradually to lipid drop during differentiated into the phase of immature and mature adipocyte.ConclusionIt is easy to detect the MSC differentiated into the phase of immature and mature adipocyte with the specific morphological change of lipid droplet, while in the phase of preadipocyte and adipoblast, there is no special morphological change, and it may need some specific markers to detect.

Key words: bone marrow stromal cell, induction, differentiation, adipogenesis, morphology