《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 19-20.

• 专题 • 上一篇    下一篇

一种获得神经干细胞单细胞的新方法

刘佳梅; 陈东; 孟晓婷   

  1. 吉林大学白求恩医学部基础医学院组胚教研室 吉林长春市 130021
  • 收稿日期:2003-12-12 出版日期:2004-01-25 发布日期:2004-01-25

A new method for isolation of neural stem cells

LIU Jia-mei,CHEN dong, MENG Xiao-ting   

  1. Department of Histology and Embryology, School of Basic Medical Science, Jilin University, Changchun 130021, Jilin, China
  • Received:2003-12-12 Published:2004-01-25 Online:2004-01-25

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摘要： 目的寻找一种有效分离神经干细胞球以获得单个神经干细胞的方法。方法应用不同浓度的胰蛋白酶、EDTA及组织培养用酵素(DisPase)分别对神经干细胞进行消化,免疫组织化学技术鉴定其分化。结果应用DisPase消化神经球,可以得到大量单个神经干细胞,且浓度以2000U/ml为佳;而应用0.25%胰蛋白酶、0.25%胰蛋白酶/0.02%EDTA混合液消化神经干细胞球则得不到大量状态较好的单细胞。结论应用DisPase消化神经干细胞球是一种理想的获得神经干细胞单细胞的好方法。

关键词: 神经干细胞, 组织培养用酵素(Dispase), 分化

Abstract: ObjectiveTo investigate an effective method to isolate neural stem cells(NSCs).MethodsNSCs were dissociated by digestion with trypsin, EDTA and different doses of Dispase,and serum-free culture techniques and immunohistochemistry techniques were used to verifying the dissociated.ResultsA lot of single neural stem cells were obtained by using Dispase to digest neurosphere, and the cells could keep its structure and morphology.ConclusionIt is an ideal method by using Dispase to digest neurosphere for isolating NSCs.

Key words: neural stem cells(NSCs), Dispase, dissociation