《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 28-30.

• 基础研究 • 上一篇    下一篇

壳聚糖生物活性载体植入大鼠脑皮质后的生物相容性研究

高尔静; 刘丙方; 鲁强; 刘玉军; 蔡青; 李晓光   

  1. 首都医科大学北京神经科学研究所,北京市神经修复重点实验室 北京市 100054
  • 收稿日期:2003-11-11 出版日期:2004-01-25 发布日期:2004-01-25

Biocompatibility of Injured Nerve Regenerated Materid following experimental traumatic brain injury in rats cortex

GAO Er-jing,LIU Bing-fang,LU Qiang,et al   

  1. Beijing Institute of Neuroscience, Capital University of Medical Science,Beijing 100054, China
  • Received:2003-11-11 Published:2004-01-25 Online:2004-01-25

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被引次数

10

摘要： 目的研究一种具有诱导神经再生功能的壳聚糖生物活性微载体(INRM)的生物相容性。方法将INRM植入到大鼠脑皮层,分别在植入材料后1周、1个月、2个月行尼氏染色、GFAP及OX42免疫组化染色,观察移入INRM周围脑的组织形态学变化。结果植入材料组的GFAP阳性细胞数量和分布在各个时间段与对照组没有显著性差异,而OX42阳性细胞数量在植入材料后与对照组相比减少。结论INRM在动物脑内具有良好的生物相容性,是一种有前景的可应用于组织工程修复的材料。

关键词: 生物相容性, 组织工程, 脑损伤, 壳聚糖, 大鼠

Abstract: ObjectiveTo evaluate the biocompatibility of a kind of scaffolding material,Injured Nerve Regenerated Materid(INRM), which play the roles of regeneration of nerve after traumatic brain injury.MethodsINRM scaffolding material were transplanted into cortex of rats after traumatic injury.The brain coronal sections were stained for Nissel, astrocyte and microglia at 1 week, 1 month, and 2 months after injury.ResultsThe presence of INRM did not alter patterns of astrocyte compared with the control group (detected with antibodies against GFAP) at any time point; but decreased the expression of microglias (detected with antibodies against OX42) compared with the control group.ConclusionThe biomaterial INRM is well suited as a biocompatible scaffold material for the repair of brain injury in the brain.

Key words: biocompatibility, tissue engineering, brain injury, chitosan, rats