《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 31-33.

• 基础研究 • 上一篇    下一篇

8Hz次声对大鼠体重及胃十二指肠5-HT表达的影响

谭永霞1;李玲1;陈景藻1;刘朝晖2   

  1. 1.第四军医大学西京医院康复理疗科 陕西西安市 710032;2.第四军医大学军事预防医学系 陕西西安市 710032
  • 收稿日期:2003-10-25 出版日期:2004-01-25 发布日期:2004-01-25

Effects of infrasound on weight and expression of 5-HT in stomach and duodenum of rats

TAN Yong-xia, LI Ling, CHEN Jing-zao,et al   

  1. Department of Rehabilitation & Physiotherapy, Xijing Hospital, Fourth Military Medical University, Xi’an 710032,Shanxi,China
  • Received:2003-10-25 Published:2004-01-25 Online:2004-01-25

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摘要： 目的探讨8Hz,90dB、130dB次声对SD大鼠体重的影响及其可能机制。方法30只雄性SD大鼠按体重随机分为对照组,8Hz、90dB及8Hz、130dB组3组。实验组分别暴露于8Hz、90dB或 8Hz、130dB次声仓中,每日作用时间2小时,共42天。对照组每日置次声仓中,但不予次声作用,所有动物每3天称体重1次。另75只随机分为对照组和8Hz,90dB、130dB的7、14、21、28、35天组共15组,每组5只,按组别予以不同时间及强度的次声作用,对照组每日置次声仓中,但不予次声作用。最后一次从次声仓取出后立即取胃及十二指肠(包括体重实验组各5只),免疫组织化学染色显示其5-羟色胺(5-HT)含量。光学显微镜下计数胃窦及十二指肠 5-HT阳性细胞数。结果实验组大鼠体重增长均较对照组缓慢(P=0.000),其中130dB组对大鼠体重增长较90dB组增长缓慢(P=0.000);实验组动物胃窦及十二指肠5-HT含量较对照组增多,以90dB的35天和130dB的28天明显(P<0.01)。结论 8Hz、90dB及 8Hz、130dB次声对雄性SD大鼠体重的增长有抑制作用,其机制可能与胃及十二指肠5-HT增多有关。

关键词: 次声, 大鼠, 体重, 胃, 十二指肠, 5-羟色胺(5-HT)

Abstract: ObjectiveTo study the effects of 8Hz,90dB and 130dB infrasound on the weight of SD rats and its possible mechanism.Methods30 male SD rats were randomly divided into the control group, 8Hz-90dB and 8Hz-130dB groups according to their original weights. The experimental groups exposed to infrasound of 8Hz-90dB or 8Hz-130dB 2 hours every day, and the control group was placed in the infrasonic storehouse without infrasound exposure. The experiment lasted for 42 days. All the rats were weighed up every 3 days.Other 75 rats were randomly divided into 7,14,21,28,35 days of control group and 8Hz-90dB and 130dB infrasound groups.Each group was given different parameter of infrasound.Then the stomach and duodenum was taken and given immunohistochemistrical straining for 5-HT(including another 15 rats of the weight group). The content of 5-HT in stomach sinus and duodenum was detected under an optical microscope.ResultsThe weight of rats exposed to infrasound increased slower than that of the control(P=0.000);a magnificent difference was observed between 90dB and 130dB group(P=0.000).Rats exposed to 130dB infrasound gained less weights than that exposed to 90dB. The infrasound group expressed more 5-HT in stomach sinus and duodenum,and the peaks were at the 28th day of 8Hz-130dB group and the 35th day of 8Hz-90dB group(P<0.01).ConclusionInfrasound of 8Hz-90dB and 8Hz-130dB can restrain the increase of weight of rats, which possibly related to the increase of 5-HT in stomach and duodenum.

Key words: infrasound, rats, weight, stomach, duodenum, 5-hydroxy-tryptamine(5-HT)