《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (01): 41-42.

• 基础研究 • 上一篇    下一篇

IL-6在视网膜脱离及复位状态下的表达

卢华1; 马志中2; 曹立群1; 刘敬1   

  1. 1.中国人民解放军解放军总医院 北京市 100853;2.北京大学第三医院眼科中心 北京市 100083
  • 收稿日期:2003-09-27 出版日期:2004-01-25 发布日期:2004-01-25

Change of IL-6 in detached and reattached retina

LU Hua, MA Zhi-zhong, CAO Li-qun, et al   

  1. General Hospital of PLA, Beijing 100853, China
  • Received:2003-09-27 Published:2004-01-25 Online:2004-01-25

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摘要： 目的探讨IL-6在视网膜脱离及复位状态下的表达。方法在SD大鼠视网膜下注入HealonGV(1.4%透明脂酸钠)制备视网膜脱离模型。放免检测脱离后不同时间点及复位后神经网膜的IL-6蛋白含量,并通过免疫组化定位IL-6的表达。应用图象分析系统分析脱离区、未脱离区及复位区色素上皮IL-6表达的差异,并对不同脱离状态的视网膜进行组织病理学分析。结果单纯色素上皮与神经网膜脱离引起网膜下的增殖,增殖在脱离后第10d达到高峰,然后下降;IL-6在色素上皮及神经网膜都有表达,神经网膜的表达在视网膜复位后下降;脱离区色素上皮IL-6的表达比未脱离区明显增高。结论IL-6积极参与了色素上皮与神经网膜脱离后的损伤修复反应;视网膜的复位可以降低IL-6的表达。

关键词: IL-6, 视网膜脱离, 视网膜复位

Abstract: ObjectiveTo determine the source of IL-6 in detached retina and the change of IL-6 level in detached and reattached retina.MethodsRetinas of SD rat were examined after subretinal injection of 1.4% Healon GV at different period of time, and the level of IL-6 in detached and reattached retina were detected by radio-immune histochemistry method. Wax-embeded sections were labeled with IL-6 antibody to determine the location of IL-6.ResultsDetached retina with normal vitreous and inner limiting membrane could only induce the subretinal fibrosis. This kind of fibrosis reached to its peak at 10th day and then remolded with time. Retinal pigment epithelial (RPE) cell, Muller cell, endothelial cell, glial cell were labled with IL-6, and the level of IL-6 in neuro-retina reached to its peak at 3rd-4th day and then downed to normal within a few days. The level of IL-6 in reattached retina was lower than in detached retina. The expression of IL-6 in RPE of detached area was stronger than in attached area.ConclusionIL-6 takes active part in wound healing process induced by the separation of RPE and neuro-retina. Reattachment can lower the expression of IL-6 in retina.

Key words: IL-6, retinal detachment, retinalreattchment