《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (03): 165-166.

• 基础研究 • 上一篇    下一篇

肿瘤坏死因子α抗体对大鼠胰性脑病保护作用的实验研究

杨雁灵1; 徐小平2; 窦科峰1; 李开宗1   

  1. 1.第四军医大学西京医院肝胆外科 陕西西安市 710032;2.第一军医大学珠江医院普通外科 广东广州市 510282
  • 收稿日期:2003-09-05 出版日期:2004-03-25 发布日期:2004-03-25

Protective effects of tumor necrosis factor-α antibody on pancreatic encephalopathy in rats

YANG Yan-ling, XU Xiao-ping, DOU Ke-feng,et al   

  1. Department of Hepatobiliary Surgery, XijingHospital, Fourth Military Medical University, Xi’an 710032,Shanxi,China
  • Received:2003-09-05 Published:2004-03-25 Online:2004-03-25

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摘要： 目的探讨肿瘤坏死因子α(TNF-α)抗体对大鼠急性出血坏死性胰腺炎时对脑组织损害的保护作用。方法将60只SD大鼠随机分为正常对照组,急性出血坏死性胰腺炎诱导组和TNF- α抗体处理组,经胰胆管逆行注射5%牛磺胆酸建立大鼠急性出血坏死性胰腺炎模型,测定血TNF-α水平,12h后处死动物,检查胰腺组织形态学变化,并检测脑组织含水量、脑微血管内白细胞聚集及附壁现象。结果经TNF-α抗体处理后,血清中TNF-α水平、脑组织含水量、脑微血管内白细胞聚集及附壁现象降低(P<0.05),胰腺组织病理损害程度减轻。结论TNF-α抗体可以减轻急性出血坏死性胰腺炎时脑损害的发生和发展。

关键词: 胰腺炎, 肿瘤坏死因子α, 胰性脑病, 大鼠

Abstract: ObjectiveTo study the protective effects of tumor necrosis factor-α(TNF-α) antibody on pancreatic encephalopathy in rats. MethodsSixty SD rats were randomly divided into the normal control group, acute necrotizing pancreatitis induction group and TNF-α antibody treated group. Acute hemorrhage necrotizing pancreatitis model in rats were induced by retrograde injection of 5% sodium taurocholate into the pancreatobiliary duct. Serum TNF-α was detected and animals were killed 12 h after drug administration. The changes of brain water contents, leucocyte accumulation and adhesion were measured, and pathological studies of pancreas and brain were performed. ResultsIn group of TNF-α antibody treated, serum TNF-α level decreased, brain water contents and leucocytes accumulation and adhension decreased significantly than that of acute necrotizing pancreatitis induction group (P<0.05). The histopathological change of pancrea was alleviative. ConclusionTNF-α antibody can alleviate the brain damage in acute hemorrhage necrotizing pancreatitis.

Key words: pancreatitis, tumor necrosis factor-α(TNF-α), pancreatic encephalopathy, rats