《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (05): 279-280.

• 基础研究 • 上一篇    下一篇

膀胱肿瘤6号染色体长臂的杂合性缺失研究

成凡1,2; 楚雍烈1; 贺大林3; 杨林3; 陈萍1; 杨娥1; 刘湘1   

  1. 1.西安交通大学医学院微生物教研室 陕西西安市 710061;2.陕西医学高等专科学校生化教研室 陕西西安市 710068;3.西安交通大学第一附属医院泌尿外科 陕西西安市 710061
  • 收稿日期:2004-03-08 出版日期:2004-05-25 发布日期:2004-05-25

Loss of heterozygosity of microsatellite DNA on 6q in bladder tumor

CHENG Fan, CHU Yong-lie, HE Da-lin, et al   

  1. Department of Microbiology, Medical College of Xi'an Jiaotong University, Xi'an 710068,Shaanxi,China
  • Received:2004-03-08 Published:2004-05-25 Online:2004-05-25

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摘要： 目的探讨6号染色体长臂的杂合性缺失(LOH)与膀胱肿瘤的关系。方法应用6q21区域附近D6S404、D6S434微卫星标志,以PCR-SSLP银染法对31例膀胱肿瘤患者的肿瘤组织进行杂合性缺失的研究。结果膀胱肿瘤组织D6S40 4LOH发生率为35.5%,D6S43 4LOH发生率为22.6%。结论6q21区域附近可能存在与膀胱肿瘤相关的肿瘤抑制基因。

关键词: 膀胱肿瘤, 杂合性缺失, 6号染色体长臂(6q)

Abstract: ObjectiveTo investigate the loss of heterozygosity (LOH) on 6q in bladder tumor.MethodsD6S404 and D6434 microsatellite markers near 6q21 were tested by PCR-SSLP-stain method on tumor DNA from 31 cases of bladder tumor.ResultsAmong these 31 cases of bladder tumor,LOH was detected in tumor tissues on site for D6S404 (35.5%) and D6S434(22.6%).ConclusionOne or more tumor suppressor gene near 6q21 maybe relevant for the development of bladder tumor.

Key words: bladder tumor, loss of heterozygosity (LOH), long arm of chromosome 6(6q)