《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (06): 348-349.

• 基础研究 • 上一篇    下一篇

骨髓来源的血管内皮祖细胞移植治疗实验性大鼠股动脉闭塞症观察

梁雪梅; 马学毅   

  1. 解放军第304医院内分泌科 北京市 100037
  • 收稿日期:2004-06-09 出版日期:2004-06-25 发布日期:2004-06-25

Treatment of experimental femoral artery obliterans in rat by transplantation with endothelial progenitor cells from bone marrow

LIANG Xue-mei, MA Xue-yi   

  1. Department of Endocrinology, PLA 304 th Hospital, Beijing 100037, China
  • Received:2004-06-09 Published:2004-06-25 Online:2004-06-25

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被引次数

26

摘要： 目的观察骨髓来源的血管内皮祖细胞(EPC)移植治疗实验性大鼠股动脉闭塞症的可行性与疗效。方法建立实验性股动脉闭塞大鼠模型。分离纯化雄性Wistar大鼠骨髓间充质干细胞(MSCs),经体外培养、扩增并诱导分化、体外BrdU标记后移植到16只模型大鼠的右侧后肢(A组),同体左侧相同部位注射生理盐水作为对照(B组)。分别在移植当天、移植后第 14天和第28天行双下肢激光多普勒扫描,并分别于移植后第14天(8只)和第28天(8只)处死动物取双下肢肌肉组织,行免疫组织化学(FⅧ、BrdU)检查。结果移植后第14天与移植当天、移植后第28天与移植后第14天相比,A组移植后下肢血流量增加(P<0.05);A组与B组相比,血流量亦增加(P<0.05)。A组肌束间血管内皮细胞呈BrdU阳性染色,而B组则为阴性。平均每视野FⅧ染色阳性的毛细血管数目A组明显多于B组(P<0.01)。结论骨髓来源的EPC移植可增加缺血部位血流量和毛细血管的密度。

关键词: 血管内皮祖细胞, 细胞移植, 股动脉闭塞症, 骨髓间充质干细胞

Abstract: ObjectiveTo observe the treatment possibility and effects on rats models with experimental femoral artery obliterans(EFAO) by transplantation of endothelial progenitor cells(EPC) from bone marrow. MethodsEFAO rat models were successfully made. Bone marrow mesenchymal stem cells(MSCs) were isolated from allogeneic male Wistar rat,cultured in DMEM in vitro. EGF,bFGF and IGF-1 were added into culture medium on the 10th day and the active EPC were labled with BrdU. 5×106 of the above cells were transplanted into the right hindlimbs i.m. as Group A in the 16 rats,and the same volume of normal saline into the opposite as Group B as control. Laser Doppler perfusion imaging was taken on the 0 day、the 14th day and the 28th day after transplantation. On the 14 day and the 28 day 8 rats were sacrificed respectively and muscles of all hindlimbs were extracted for immunohistochemical examinations (by FⅧ and BrdU).Results1. In group A the skin blood perfusion in hindlimb were significantly increased as compared with group B(P<0.05). 2. Some positive stained EPC by BrdU were found in Group A but not in Group B. 3. The numbers of blood vessels with positive staining of FⅧ in hindlimb on the 28 day in Group A were more obviously than that in Group B (P<0.01). ConclusionTransplantation of EPC from bone marrow can significantly increase the skin blood perfusion and capillary density in ischemic hindlimbs in EFAOrats.

Key words: endothelial progenitor cells(EPC), cell transplantation, femoral artery obliterans, bone marrow mesenchymal stem cells(MSCs)