《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (07): 406-407.

• 基础研究 • 上一篇    下一篇

小分子抗自由基化合物对大鼠脑损伤保护作用研究

郭金兰1; 张澄波2   

  1. 1.中国康复医学研究所 北京市 100068;2.首都医科大学生化教研室 北京市 100054
  • 收稿日期:2003-11-19 出版日期:2004-07-25 发布日期:2004-07-25

Protective effects of the compound with low molecular weight of anti-free radical on brain injury in rats

GUO Jin-lan, ZHANG Cheng-bo   

  1. Chinese Rehabilitation Medicine Research Institute, Beijing 100068, China
  • Received:2003-11-19 Published:2004-07-25 Online:2004-07-25

PDF (PC)

被引次数

33

摘要： 目的观察小分子抗自由基化合物对大鼠脑缺血、缺氧损伤的保护作用,为临床应用提供依据。方法采用结扎大鼠颈动脉及进入低氧仓的方法建立脑缺血、缺氧损伤模型;腹腔注射小分子抗自由基化合物,观察大鼠脑组织海马区域细胞形态变化及血清中超氧化物歧化酶(SOD)及丙二醛(MDA)的变化情况。结果与模型对照组相比,实验组大鼠脑组织海马CA1区锥体细胞排列整齐紧密,形态基本正常,仅偶见细胞退行性改变;血清SOD酶活力升高,MDA含量降低(P<0.05)。结论小分子抗自由基化合物能提高大鼠血清中SOD酶活力 ,清除自由基,抑制脂质过氧化,保护脑细胞免受缺血、缺氧损害。

关键词: 自由基, 脑损伤, 超氧化物歧化酶, 丙二醛

Abstract: Objective To study the protective effects of the compound with low molecular weight of anti free radical on brain ischemic and hypoxic injuries in rats.Methods The model of brain ischemic and hypoxic injuries in rats was established by unilateral carotid artery ligature just for 2 h. The compound was injected 30 minutes after the rats act on hypoxic state (10% O2+90%N2) for 1 h. Then the serum of rats was separated and the value of the superoxide dismutase (SOD) activity and malondiade (MDA) were determined, and the pathological changes were observed in brain tissue.Results Compared with rats in the model group, the compound decreased the concentrations of MDA in serum (P<0.05), and raised the level of SOD (P<0.05) significantly in therapy group. Otherwise, the pyramidal cells remained organized order, with only a few cells degenerated occasionally in rats of therapy group. Conclusion The compound has SOD like activity. It can scavenge the free radical and inhibit peroxidation of lipid, so it can prevent brain cells from the damage.

Key words: radical, brain injury, superoxide dismutase (SOD), malondiade