《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (08): 455-457.

• 基础研究 • 上一篇    下一篇

常温细胞长期生存装置在兔间充质干细胞无源搭载太空飞行中的应用

张岚1; 蔡哲1; 房青2; 向青2; 李红艳2; 徐梅2; 徐波2; 唐劲天2; 孔晶3; 刘国玲3; 高福云3   

  1. 1.中日友好医院临床医学研究所免疫学—组织工程学研究室 北京市 100029;2.中日友好医院临床医学研究所肿瘤—分子生物学研究室 北京市 100029;3.中日友好医院临床医学研究所分子药物研究室 北京市 100029
  • 收稿日期:2004-07-16 出版日期:2004-08-25 发布日期:2004-08-25

Application of outer space cell living system in space flight of rabbit's mesenchymal stem cell

ZHANG Lan, CAI Zhe, FANG Qing,et al   

  1. Department of Immunology-Tissue Engineering, China-Japan Friendship Clinical Medicine Institute, Beijing 100029, China
  • Received:2004-07-16 Published:2004-08-25 Online:2004-08-25

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摘要： 目的利用常温细胞长期培养装置,探讨间充质干细胞(MSCs)无源搭载长期太空飞行的生存条件和培养方法。方法将分离扩增培养的兔MSCs分别接种于培养瓶与常温细胞长期生存装置中,在无O2 、CO2 供应,以及无更换培养基条件下,模拟太空温度变化,观察MSCs的长期培养生存状况。结果兔MSCs在无O2、CO2 供应,及无培养基更换条件下,4℃/3 7℃单层培养连续存活至少15天;太空组细胞在常温细胞生存装置中可生存30天,两者有显著性差异。结论常温细胞长期生存装置在太空飞行保温温度下可支持兔MSCs连续存活30天。

关键词: 常温细胞长期生存装置, 间充质干细胞, 太空飞行

Abstract: ObjectiveTo investigate the condition and method of a long time mesenchymal stem cell (MSCs) culture with outer space cell living system carried by a recoverable satellite.MethodsMSCs were obtained from 1 week New Zealand rabbits, and seeded to culture flask and outer space cell living system. Under the condition of absence O2, CO2 and medium unable changed, influence of monolayer culture and outer space cell living system upon survival of long time MSCs culture in simulated space various temperature was observed.ResultsUnder the condition of absence O2, CO2 and medium unable changed, monolayer cultured MSCs were maintained to survive for 15 days, and group of space MSCs were successfully maintained to survive for 30 days in outer space cell living system. There was a significant difference between two methods. ConclusionOuter space cell living system can maintain MSCs to survive for 30 days under space flight temperature.

Key words: outer space cell living system, mesenchymal stem cell (MSCs), space flight