《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (08): 458-460.

• 基础研究 • 上一篇    下一篇

大鼠骨髓基质细胞体外诱导分化为内皮细胞实验研究

王春雪1; 万虹2; 历俊华2; 王拥军1; 毛淑静3; 刘丽萍1; 翟晶2   

  1. 1.首都医科大学附属北京天坛医院神经内科 北京市 100050;2.北京市神经外科研究所 北京市 100050;3. 河北医科大学第二医院临床药理专业 河北石家庄市 050000
  • 收稿日期:2004-05-21 出版日期:2004-08-25 发布日期:2004-08-25

Multiplication and differentiation into endothelial cells of bone marrow stromal cells of rats in vitro

WANG Chun-xue, WAN Hong,LIJun-hua, et al   

  1. Department of Neurology, Beijing Tiantan Hospital, Beijing 100050, China
  • Received:2004-05-21 Published:2004-08-25 Online:2004-08-25

PDF (PC)

被引次数

7

摘要： 目的探讨骨髓基质细胞(BMSC)向内皮细胞分化的可行性。方法无菌条件下获取BMSC,实验组以400ng/ml重组人粒细胞-巨噬细胞集落刺激因子(rhGM-CSF)为诱导剂,进行体外诱导分化;对照组用1640培养液培养。8d后进行免疫组化染色进行特异性内皮细胞标志物鉴定。结果分化后的细胞在光镜下具有内皮细胞的形态学特征;免疫组化染色证实实验组诱导后的细胞为内皮细胞。结论BMSC在高浓度rhGM-CSF作用下,可以分化为内皮细胞。

关键词: 重组人粒细胞巨噬细胞集落刺激因子(rhGM-CSF), 骨髓基质细胞(BMSC), 内皮细胞, 冯威勒不兰特因子(vWF), 因子Ⅷ相关抗原(FⅧ-R Ag)

Abstract: ObjectiveTo explore the feasibility of bone marrow stromal cells (BMSC) of rats differentiating into endothelial cells.MethodsBone marrow cells were obtained by an aseptic technique. Afterwards, the obtained cells were divided into two groups: cells in test group were propagated in 1640 medium with recombinant human GM-CSF (rhGM-CSF) (400ng/ml), and that in control group were propagated in medium with 1640 medium only. The differentiated cells were detected by specific immunology marker by immunocytochemistry and immunofluorescence at day 8.ResultsThe differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy. Cells of the test groups demonstrated specific characters by immunocytochemistry stain.ConclusionBone marrow stromal cells can multiply vigorously and differentiate into cells with endothelial cells characters in the medium with high concentration rhGM-CSF.

Key words: recombinant human GM-CSF(rhGM-CSF), bone marrow stromal cells(BMSC), endothelial cell, von Willebrand factor(vWF), factor Ⅷ related antigen (FⅧ-R Ag)