《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (11): 652-653.

• 专题 • 上一篇    下一篇

低温细胞生存系统长期保存CHO细胞的初步研究

徐波; 向青; 徐梅; 房青; 李红艳; 靳耀英; 唐劲天   

  1. 中日友好医院临床医学研究所生化-分子生物研究室 北京市 100029
  • 收稿日期:2004-10-13 出版日期:2004-11-25 发布日期:2004-11-25

Preliminary study of hypothermia cell living system in CHO(dhfr-) cells culture

XU Bo,XIANG Qing,XU Mei,et al   

  1. Department of Biochemistry and Molecule Biology,Institute of Clinical Medical Science,China-Japan Friendship Hospital,100029 Beijing, China
  • Received:2004-10-13 Published:2004-11-25 Online:2004-11-25

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被引次数

6

摘要： 目的验证基因工程细胞空间搭载系统在低温(18℃-30℃、无源等条件下保存细胞的可行性,观察此系统对生物工程细胞CHO(dhfr-)生长特性的影响。方法将CHO (dhfr-)生物工程细胞株在空间搭载系统中培养25d后,利用光镜、MTT法、FCM分析、3H掺入法及染色体分析观察细胞生长特性。结果细胞低温培养25d后,CHO(dhfr-)细胞呈多种形态;细胞周期变化差异不显著;染色体无断裂;细胞生长速度显著减慢;大分子生物合成显著增加。结论空间搭载系统搭载CHO(dhfr-)细胞进入空间后,虽然细胞产生了许多生理性变化,但能使CHO(dhfr-)细胞生存下来。利用空间搭载系统搭载细胞是完全可行的。

关键词: 低温, 空间搭载系统, CHO(dhfr-)细胞, 生长特性

Abstract: ObjectiveTo validate the feasibility of the outer space carrying system in culturing the CHO(dhfr-)cells in hypothermia, and to observe the effects of this carrying system on the growth characteristics of the CHO(dhfr-) cells.MethodsThe growth characteristics of the CHO(dhfr-) cells were observed and analyzed with cell morphological observation, MTT assay, FCM,3H incorporation and chromosome after the cells were cultured for 25 days in the carrying system. ResultsComparing with the control group, the CHO(dhfr-) cells appeared multiple cell morphological changes, the difference of cell cycle was not significant, the broken chromosome was not seen, the cell growth speed decreased markedly and big molecular biosynthesis increased obviously. ConclusionThe outer space carrying system has no outstanding effects on the survival and heredity of the CHO(dhfr-) cells, so that it can be used in cell carry.

Key words: hypothermia, outer space carrying system, CHO(dhfr-) cell, growth characteristics