《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (11): 660-661.

• 基础研究 • 上一篇    下一篇

Schwann细胞与筋膜共同培养的实验研究

闫长祥1; 万虹2; 历俊华2; 于春江1; 王忠诚2   

  1. 1.首都医科大学附属复兴医院北京三博复兴脑科医院神经外科 北京市 100038;2.北京市神经外科研究所 北京市 100050
  • 收稿日期:2004-07-19 出版日期:2004-11-25 发布日期:2004-11-25

Co-culture of Schwann cell with fascia

YAN Chang-xiang, WAN Hong, LI Jun-hua, et al   

  1. Department of Neurosurgery, Beijing Sanbo Fuxing Neurosurgery Hospital, Fuxing Hospital, Capital University of Medical Sciences, Beijing 100038, China
  • Received:2004-07-19 Published:2004-11-25 Online:2004-11-25

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摘要： 目的探讨SchWann细胞与筋膜共同培养的方法,为神经缺损修复提供实验基础。方法将SchWann细胞与筋膜共同培养,免疫组织化学双染、相差显微镜连续观察及间接免疫荧光染色显示SchWann细胞胶质特异性标记蛋白S-100表达和BrdU掺入。结果相差显微镜下可见筋膜周围有大量SchWann细胞,且生长迅速,胞体呈梭形,核为椭圆形,胞体两侧有两个细长的突起,并呈平行排列,少见成纤维细胞。形态学连续观察和S-100间接免疫荧光染色都显示BrdU掺入。筋膜BrdU和S-100免疫组织化学双染可见筋膜上有大量的BrdU和S-100双染阳性的SchWann细胞,胞核为深蓝色,胞浆红褐色。结论在筋膜上有大量具有增殖活性的SchWann细胞,该筋膜可望用于修复神经缺损。

关键词: Schwann细胞, 筋膜, 体外培养

Abstract: ObjectiveTo explore the method of co-culture of Schwann cell(SCs) with fascia and provide experimental basis for repairing transected nerve.MethodsSCs were co-cultured with fascia.Double staining by anti-BrdU and anti-S-100,S-100 fluorescent staining and anti-BrdU staining were used.ResultsThere were a plenty of SCs around fascia proliferated rapidly and disposed in parallel. SCs could be distinguished from fibroblastic cells by S-100 fluorescent staining and also be staining positive by anti-BrdU antibody,implying their high proliferous ability. Anti-BrdU and anti-S-100 staining showed numerous double staining positive SCs on the fascia: nucleus was stained deep blue while cytoplasm was stained red.ConclusionMany SCs with high proliferous ability were seen on the fascia, which can be used to repair transected nerve.

Key words: Schwann cell, fascia, culture in vitro