《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (12): 726-728.

• 专题 • 上一篇    下一篇

高压氧对成年大鼠脑梗死灶体积和基质金属蛋白酶的影响

潘钰1; 张朝东2   

  1. 1.首都医科大学康复医学院 北京市 100068;2.中国医科大学第一临床学院神经内科 辽宁沈阳市 110001
  • 收稿日期:2004-06-03 出版日期:2004-12-25 发布日期:2004-12-25

Effect of hyperbric oxygen on infarct volume and matrix metalloproteinase after permanent focal cerebral ischemia in adult rats

PAN Yu, ZHANG Chao-dong   

  1. Faculty of Rehabilitaion of Capital University of Medical Sciences, Beijing 100068, China
  • Received:2004-06-03 Published:2004-12-25 Online:2004-12-25

PDF (PC)

被引次数

37

摘要： 目的探讨高压氧(HBO)对成年大鼠持续性局灶脑缺血梗死灶体积的影响及其可能的作用机制。方法应用血管内细丝线栓堵大脑中动脉(MCA)制作持续性脑缺血大鼠模型,用HBO(2.0ATA)治疗后,观察MCA缺血后6h、24h、48h、72h、120h和10d各组大鼠脑梗死灶体积、基质金属蛋白酶(matrix metalloProteinase,MMP)MMP-2和MMP-9的变化。结果与缺血组相比,HBO组大鼠120h和10d梗死灶体积减小,缺血48—120hMMP-9蛋白表达减少,MMP-2蛋白表达无明显变化。结论HBO能减小脑缺血梗死灶体积,其作用可能与HBO下调MMP-9蛋白表达有关。

关键词: 高压氧, 局灶性脑缺血, 基质金属蛋白酶(matrix metalloproteinase, MMP)

Abstract: ObjectiveTo investigate the effect of hyperbric oxygen (HBO) on infarct volume and relevant mechanism after permanent focal cerebral ischemia in adult rats.MethodsRat model of focal cerebral ischemia induced by intraluminal filament occlusion of middle cerebral artery (MCA) was used. HBO(2.0 ATA) was applied to HBO group. Infarct volume, matrix metalloproteinase 2 (MMP-2) and MMP-9 were detected at 6h, 24h, 48h, 72h,120h and 10d after ischemia.ResultsThe infarct volume obviously decreased at 120h and 10d and expression of MMP-9 lowered at 48—120h in HBO groups. There was no significant change in MMP-2.Conclusion HBO can reduce infarct volume after cerebral ischemia, which may be related to downregulation of MMP-9 levels.

Key words: hyperbric oxygen (HBO), focal cerebral ischemia, matrix metalloproteinase (MMP)