《中国康复理论与实践》

《中国康复理论与实践》 ›› 2004, Vol. 10 ›› Issue (12): 754-755.

• 基础研究 • 上一篇    下一篇

TRAIL联合化疗药物对人宫颈癌Hela细胞增殖与凋亡的影响

张佳冰1; 张银刚2   

  1. 1.海南医学院药学系化学教研室 海南海口市 571101;2.西安交通大学医学院地方病研究所 陕西西安市 710061
  • 收稿日期:2004-03-15 出版日期:2004-12-25 发布日期:2004-12-25

Effect of TRAIL and it's combination with chemotherapeutic agents on the proliferation and apoptosis of cervical cancer

ZHANG Jiabing, ZHANG Yin-gang   

  1. Chemical Department, Hainan Medical College, Haikou 571101, Hainan, China
  • Received:2004-03-15 Published:2004-12-25 Online:2004-12-25

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摘要： 目的探讨肿瘤坏死因子相关诱导凋亡配体(TRAIL)对人宫颈癌Hela细胞的作用,以及与化疗药物的协同作用。方法将Hela细胞接种至96孔培养板后分别加入浓度为l.0、10.0、10 0.0μl/L的TRAIL、0.1、1.0、10.0mg/L的阿霉素(ADM)和丝裂霉素(MMC),及不同匹配的TRAIL+MMC和TRAIL+ADM,采用四甲基偶氮唑盐(MTT)比色法分别检测肿瘤细胞的生存率;将Hela细胞接种至12孔板,培育24h后加入不同浓度的TRAIL、ADM、MMC、TRAIL+ADM、TRAL+MMC,用流式细胞术检测不同处理组肿瘤细胞的凋亡率和死亡率。结果100μg/LTRAIL引起细胞的凋亡率为20.1%,与无药物组1.1%的凋亡率有非常显著性差异(P<0.01);单独采用10mg/LMMC、ADM对细胞的抑制率为36.0%和44.1%,而100μg/LTRAIL与 10mg/LMMC、ADM联合后对细胞的抑制率分别达到58.4%和73.7%,两者有协同作用(P<0.05)。结论在体外实验中,TRAIL可通过诱导宫颈癌Hela细胞的凋亡而产生抗肿瘤作用;TRAIL与化疗药物ADM、MMC有协同抗肿瘤作用。

关键词: 宫颈癌, 化疗, 肿瘤坏死因子相关诱导凋亡配体

Abstract: ObjectiveTo explore the role of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and it's combination with chemotherapeutic agents in treatment of cervical cancer.Methods 100μl of cervical cancer cells suspension were added to each well of 96 well plates. Cells were incubated for 24 hours with TRAIL, Adriamycin (ADM), Mitomycin C (MMC) and TRAIL combined with chemotherapeutic agents at different concentration. Rates of apoptosis and death of cells were detected by flow cytometry.ResultsRates of apoptosis of cells were 20.1% by treated with 100 μg/L TRAIL singly, which was significantly different compared with groups without agents (P<0.01). Inhibitory rates were 36.0% by treated with 10.0mg/L MMC, 44.1% by ADM. However inhibitory rates reached 58.4% or 73.7% by 100.0 μg/L TRAIL +10.0 mg/L MMC or ADM. Combined of TRAIL and MMC, TRAIL and ADM resulted in a synergistic cytotoxic effect (P<0.05).ConclusionIn vitro, TRAIL is an agent for anti cervical cancer by inducing the apoptosis of tumor cell; TRAIL can enhance the effect of ADM and MMC.

Key words: cervical cancer, chemotherapy, tumor necrosis factor related apoptosis inducing ligand (TRAIL)