《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (01): 10-12.

• 专题 • 上一篇    下一篇

移植神经干细胞对血管性痴呆大鼠海马胆碱能神经元的影响

叶建新1; 王玮2; 郑志3   

  1. 1.南京军区福州总医院神经内科 福建福州市 350025;2.福建医科大学解剖学与组织胚胎学系 福建福州市 350004;3.福建医科大学分子医学研究中心 福建福州市 350004
  • 收稿日期:2004-11-01 出版日期:2005-01-25 发布日期:2005-01-25

Effect of transplantation of the neural stem cells on the cholinergic neurons in hippocampus of vascular dementia rats

YE Jian-xin,WANG Wei, ZHENG Zhi-hong   

  1. Department of Neurology, Fuzhou Military General Hospital of Nanking Army Area, Fuzhou 350025, China
  • Received:2004-11-01 Published:2005-01-25 Online:2005-01-25

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摘要： 目的研究神经干细胞移植后血管性痴呆(VD)大鼠海马胆碱能神经元的变化。方法制作VD大鼠模型,随机取用VD大鼠模型12只,分移植组6只,痴呆组6只。另外,取假手术组6只。新生大鼠脊髓神经干细胞经分离培养和纯化,移植于大鼠海马。术后8周 ,免疫组织化学及荧光染色检测神经干细胞能否存活、迁移及3组大鼠海马CA1区胆碱能神经元数目的变化。结果移植组大鼠海马CA1区胆碱能神经元数目较痴呆组明显增多。结论神经干细胞移植后能迁移至海马,分化或诱导海马产生具有ChAT活性神经元,所产生的ChAT活性神经元可能就是胆碱能神经元。

关键词: 神经干细胞, 移植, 血管性痴呆, 海马, 胆碱能神经元, 大鼠

Abstract: ObjectiveTo study the change of cholinergic neurons of hippocampus of vascular dementia rats after transplantation of the neural stem cells(NSCs) in hippocampus.MethodsAfter the vascular dementia model was reproduced,the rats were randomly divided into transplantation,dementia and pseudo-operative groups.8 weeks after operation,immunofluorescence was used to observe the survivors and migration of neural stem cells.Immunochemistry staining was used to observe the number of ChAT positive neurons in hippocampus.ResultsThe number of the ChAT positive neurons in the CA1 subfield of the hippocampus were more significantly increased in transplantation group than that in dementia group. ConclusionChAT positive neurons can be found in hippocampus area of vascular dementia rats after transplanting NSCs into the hippocampus, which seem to be cholinergic neurons.

Key words: neural stem cells, transplantation, vascular dementia, hippocampus, cholinergic neurons, rats