《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (02): 81-83.

• 专题 •    下一篇

硫酸镁对兔脊髓缺血再灌注损伤的保护作用

樊立宏; 程斌; 王坤正; 李勇   

  1. 西安交通大学第二医院骨科 陕西西安市 710004
  • 收稿日期:2004-08-13 出版日期:2005-02-25 发布日期:2005-02-25

Effects of magnesium sulfate on spinal cord ischemia-reperfusion injury in rabbits

FAN Li-hong, CHENG Bin, WANG Kun-zheng,et al   

  1. Department of Orthopedics, Second Affiliated Hospital of Xi'an Jiao Tong University, Xi'an 710004, Shanxi, China
  • Received:2004-08-13 Published:2005-02-25 Online:2005-02-25

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摘要： 目的 观察硫酸镁对兔脊髓缺血再灌注损伤的保护作用。方法 将27只新西兰大白兔随机分为 A组(硫酸镁处理组)、B组(生理盐水组)和C组(假手术),每组9只。夹闭腹主动脉肾下段30 min后恢复血流再灌注 48 h,建立兔脊髓腰骶段缺血模型。A组给予静脉硫酸镁灌注(0.25 ml//kg/h);B组用等量生理盐水代替;C组仅行中线剖腹术,不结扎动脉。在缺血前、缺血30 min及再灌注后60 min对动物行体感诱发电位(SEP)监测;再灌注24 h及48 h后对A、B组动物行运动功能评分;再灌注后48 h处死动物,对脊髓行组织病理学检查。结果 C组SEP无明显变化,动物均完全康复。缺血 30 min时,B组 SEP波形消失,A组波幅降为基线的29%;再灌注60 min后A组、B组SEP波幅分别渐升至基线的74%和49%。再灌注60 min时,A组 SEP的 N1、P1波峰潜伏期分别为(28.9±1.9)ms和(57.3±3.2)ms,与缺血前及B组相比均有显著性差异(P<0.05);再灌注24h和48 h,A组的神经功能评分均高于B组( P<0.05);再灌注48 hA组的脊髓前角神经细胞计数明显高于B组(P<0.01)。结论 硫酸镁具有减轻兔脊髓缺血再灌注损伤及保护神经功能的作用。

关键词: 硫酸镁, 脊髓损伤, 缺血再灌注, 体感诱发电位

Abstract: ObjectiveTo observe effects of intravenous magnesium sulfate (MgSO 4) administration on ischemia reperfusion injury of the spinal cord in rabbits.MethodsNew Zealand White rabbits (n=27) were randomly divided into the group A (treated with MgSO4), group B (treated with saline) and group C (sham group) with 9 animals in each group. Ischemic model was established with midline laparotomy and clamping the aorta just distal to left renal artery and proximal to aortic bifurcation for 30 min followed by a reperfusion period of 48 h. Animals were treated with 0.25 ml/kg/h MgSO4 intravenous infusion in group A, treated with similar volume of saline as control in group B, and were anesthetized and subjected laparotomy without aortic occlusion in group C. Somatosensory evoked potentials (SEP) were monitored before ischemia, during ischemia and in the first 60 min of reperfusion. The neurological outcome was clinically evaluated up to 48 h post ischemia, and motor function was scored. The animals were sacrificed two days post ischemia, and spinal cords were processed for histopathological examination.ResultsSEP amplitudes and latencies in group C did not change during the procedures and all animals recovered without neurological deficits. The waves disappeared in group B and reduced to 29% of the initial amplitude at the end of the ischemia in group A. After 60 min reperfusion, SEP amplitudes returned gradually to 74% and 49% of the initial amplitude respectively (P<0.01) in groups A and B. The N1, P1 latencies returned gradually to (28.9±1.9) ms, (57.3±3.2) ms in group A and (30.7±0.9) ms, (61.2±2.9) ms in group B (P<0.05). The average motor function score in group A was significantly higher than that in group B at 24 h and 48 h after reperfusion (P<0.01).ConclusionMgSO4 intravenous infusion may relieve spinal cord injury and preserve neurological function in transient spinal cord ischemia in rabbits.

Key words: magnesium sulfate, spinal cord injury, ischemia reperfusion, somatosensory evoked potentials (SEP)