《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (02): 89-90.

• 专题 • 上一篇    下一篇

大鼠完全性脊髓损伤后降钙素基因相关肽与运动终板的关系

张庆民 ; 关骅 ; 洪毅   

  1. 1.首都医科大学康复医学院 北京市 100068;2.北京博爱医院脊柱
  • 收稿日期:2005-01-28 出版日期:2005-02-25 发布日期:2005-02-25

Relationship of the change of calcitonin gene-related peptide and degeneration of the motor endplate after spinal cord injury in the rat

ZHANG Qing-min, GUAN Hua, HONG Yi   

  1. Department of Spine and Spinal Cord Surgery, Faculty of Rehabilitation of the Capital University of Medical Science, Beijing 100068, China
  • Received:2005-01-28 Published:2005-02-25 Online:2005-02-25

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摘要： 目的 观察大鼠完全性脊髓损伤后降钙素基因相关肽(CGRP)和乙酰胆碱酯酶(AChE)在骨骼肌运动终板中的变化特点及运动终板的变化。方法 采用Wistar大鼠制作T10脊髓横断模型,分别于损伤后 1、2、4、8 周取胫前肌肌腹标本,以免疫组化法检测AChE和CGRP在运动终板中的变化。结果 脊髓横断1周后CGRP在运动终板中的数量、分布即明显减少,着色变浅,而AChE在运动终板中的变化发生在损伤后4周时;运动终板中 CGRP和 AChE始终未完全消失。结论 上运动神经元损伤后骨骼肌运动终板存在退变现象;AChE和CGRP与运动终板的退变相关;检测CGRP能更早地显示运动终板的改变。

关键词: 脊髓损伤, 运动终板, 降钙素基因相关肽, 大鼠

Abstract: ObjectiveTo observe changes of calcitonin gene related peptide (CGRP) and aetylcholinesterase (AChE) in motor endplate (MEP) after complete spinal cord injury (SCI) in the rat.MethodsSCI model of Wistar rats were established by transecting T10 spinal cord completely. The venters of the tibial anterior muscle were taken at 1, 2, 4 and 8 weeks postoperatively to carry out the experiment.The changes of CGRP and AChE in the MEP were investigated with immunohistochemical test.ResultsAfter complete SCI, the quantity and distribution of CGRP decreased in MEP of 1-week sample, and the changes of AChE were observed until 4-week sample, and although the staining was light and sparse, CGRP and AChE existed in MEP during 2 months postoperatively.ConclusionThere is degeneration of MEP in skeletal muscle after upper motor neuron injury; CGRP and AChE are related to the degeneration of MEP; CGRP test can show the changes of MEP earlier.

Key words: spinal cord injury (SCI), motor endplate (MEP), calcitonin gene reated peptide (CGRP), aetylcholinesterase (AChE), rat