《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (03): 189-191.

• 基础研究 • 上一篇    下一篇

大鼠小肠缺血再灌注后氧自由基改变及肝脏Bax、Bcl-2、p53的表达

李纪鹏; 王为忠; 凌瑞; 陈冬利; 李孟彬; 刘季   

  1. 第四军医大学西京医院普通外科 陕西西安市 710032
  • 收稿日期:2004-09-28 出版日期:2005-03-25 发布日期:2005-03-25

Change of free radical in serum and the expression of Bax, Bcl-2 and p53 in the liver after ischemia-reperfusion of small intestine

LI Ji-peng,WANG Wei-zhong, LING Rui,et al   

  1. Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shanxi, China
  • Received:2004-09-28 Published:2005-03-25 Online:2005-03-25

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摘要： 目的探讨小肠缺血再灌注后对肝组织的损伤。方法建立小肠缺血再灌注模型,分对照组,再灌注后0、30min、1h、2h、1d、3d、7d共8组,于各时点检测血中一氧化氮(NO)和超氧化物歧化酶(SOD)的浓度,用免疫组织化学SP法观察肝组织中Bax、Bcl-2、P53的表达情况。结果大鼠小肠缺血再灌注后NO浓度在再灌注0min升高,但至再灌注2h时则明显降低,其后又持续升高,至再灌注7d时达高峰。SOD浓度在再灌注0min明显下降,再灌注2h时升高,随后持续下降,至再灌注7d时达最低水平。再灌注0min,肝组织中Bax、Bcl-2、P53阳性细胞率增高,再灌注30min时,Bax、Bcl-2、P53阳性细胞率升高更为明显,但Bcl-2表达明显高于Bax(P<001)。再灌注2h时,3种基因均有所降低,其后又开始升高,再灌注7d时阳性细胞率达高峰,Bax表达明显高于Bcl-2(P<001)。结论大鼠小肠缺血再灌注后引起血中NO和SOD的浓度变化及Bax、Bcl-2、P53阳性细胞在肝组织中的表达改变,并可能引起细胞调亡和损伤。

关键词: 缺血再灌注, 小肠, 一氧化氮, 超氧化物歧化酶, Bax, Bcl-2, p53, 大鼠

Abstract: ObjectiveTo study the injuries of liver after ischemia-reperfusion of small intestine of the rat.MethodsModels of ischemia-reperfusion of small intestine was made with rats. At 0 min, 30 min, 1h, 2h, 1d, 3d,7d after reperfusion, the concentration of nitric oxide(NO), superoxide dismutase (SOD) in the serum was examed and the expression of Bax, Bcl-2 and p53 in the liver was observed by the immunohistochemical SP method.ResultsThe concentration of NO increased apparently 0 min after reperfusion, but decreased 2 h after, then increased gradually to a peak at 7th day. But for SOD, the concentration decreased 0 min after reperfusion, increased 2 h after,and decreased to the lowest level at 7th day. The immunohistochemical SP positive cells were observed in sinus endothelial cells and hepatocytes. The ratio of positive cells of Bax,p53 and Bcl-2 began to increase 0 minute after reperfusion and increased continuously 30 min after, while that of Bcl-2 was higher than that of Bax(P<0.01). It decreased apparently 2h after, and then increased till 7d after reperfusion,while the ratio of Bax positive cells was higher than that of Bcl-2(P<0.01).ConclusionThe change of concentration of NO, SOD and the expression of positive cells of Bax, Bcl-2 and p53 might play a important role in apoptosis and injuries of the liver after ischemia-reperfusion of small intestine of rat.

Key words: ischemia and reperfusion, small intestine, nitric oxide (NO), superoxide dismutase (SOD), Bax, Bcl-2, p53, rat