《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (04): 273-275.

• 基础研究 • 上一篇    下一篇

N-已酰半胱氨酸对大鼠脑梗死灶体积及caspase-1蛋白表达的影响

马莉   

  1. 沈阳医学医学院附属中心医院神经内科 辽宁沈阳市 110024
  • 收稿日期:2005-01-10 出版日期:2005-04-25 发布日期:2005-04-25

Effect of N-acetylcysteine on cerebral infarct volume and expression of caspase-1 protein in rat

MA Li   

  1. Department of Neurology,Shenyang Central Hospital, Shenyang 110024, Liaoning, China
  • Received:2005-01-10 Published:2005-04-25 Online:2005-04-25

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摘要： 目的观察N-已酰半胱氨酸(NAC)对成年大鼠局灶性脑缺血后脑梗死体积及凋亡基因casPase-1表达的影响。方法70只大鼠随机分为正常对照组、假手术组、缺血组和NAC组,NAC组于大脑中动脉栓塞后30 min腹腔注射NAC 150 mg/kg,比较缺血组和NAC组的梗死灶体积、casPase-1免疫组化阳性细胞数。结果NAC组24h～7d各检测时间点的梗死灶体积小于缺血组,casPase-1免疫组化阳性细胞数少于缺血组(P<0.01)。结论NAC有减小持续性局灶性脑缺血大鼠脑梗死灶体积、抑制细胞凋亡的作用,且可能与抑制casPase-1蛋白表达有关。

关键词: 缺血性脑血管病, N-已酰半胱氨酸(NAC), 细胞凋亡

Abstract: ObjectiveTo observe the effect of N-acetylcysteine (NAC) on cerebral infarct volume and expression of caspase-1 protein after permanent focal cerebral ischemia in adult rat.Methods70 healthy adult male SD rats were randomly divided into normal control group, sham operated group, ischemia group and NAC group. NAC group treated with NAC (150 mg/kg, intraperitoneal injection) after middle cerebral artery embolized for 30 minutes. The change of infarct volume and the number of caspase-1 positive staining cells in ischemia group and NAC group was compared.ResultsThe infarct volum of NAC group decreased significantly compared with that of ischemia groups at different time-points of cerebral ischemia. In NAC group, the number of caspase-1 positive staining cells deceased at different time-points of cerebral ischemia compared with those of ischemia group.ConclusionTreatment with NAC after brain ischemia may reduce infarct volum permant focal cerebral ischemia in rat. The effect of NAC decreasing apoptosis following permanent focal cerebral ischemia may be related to downregulation of caspase-1 protein.

Key words: ischemia disease, N-acetylcysteine (NAC), cell apoptosis