《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (07): 553-554.

• 基础研究 • 上一篇    下一篇

腺苷不参与大鼠双后肢缺血预处理对移植胰的保护作用

刘小南1; 霍婷婷2; 王为忠1; 管文贤1   

  1. 1.第四军医大学西京医院胃肠外科 陕西西安市 710033;2.第四军医大学西京医院麻醉科 陕西西安市 710033
  • 收稿日期:2005-05-09 出版日期:2005-07-25 发布日期:2005-07-25

Protection of ischemic preconditioning in the posterior limbs of donor' pancreas graft without involvement adenosine in rat

LIU Xiao-Nan, HUO Ting-Ting, WANG Wei-Zhong, et al   

  1. Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, Shanxi, China
  • Received:2005-05-09 Published:2005-07-25 Online:2005-07-25

PDF (PC)

摘要： 目的探讨腺苷是否参与了大鼠双后肢缺血预处理对移植胰保护作用的信号传导机制。方法18只糖尿病大鼠随机分为缺血再灌注组(I/R组,n=6);缺血预处理组(IPC组,n=6)和缺血预处理+8环戊基1,3二丙基黄嘌呤(DPCPX)组(IPC+DPCPX组,n=6),各组大鼠均行胰腺移植。检测各组再灌注前、后血糖,再灌注后2h血清中肿瘤坏死因子(TNF)α、胰腺组织中髓过氧化酶(MPO)和丙二醛(MDA)含量,并利用TUNEL法检测移植胰凋亡细胞。结果再灌注后I/R组血糖、TNFα,胰腺组织凋亡指数(AI)、MPO和MDA含量均显著高于IPC组(P<0.01)和IPC+DPCPX组(P<0.01),IPC+DPCPX组与IPC组再灌注后各项指标无显著性差异(P>0.05)。结论大鼠双后肢肢缺血预处理对大鼠移植胰的缺血再灌注损伤具有保护作用,腺苷与这种保护作用无关。

关键词: 胰腺移植, 缺血预处理, 腺苷, 大鼠

Abstract: ObjectiveTo investigate the effect of ischemic preconditioning (IPC) in the posterior limbs of rats of donor' pancreas graft, and analyze the correlations with adenosine. Methods18 steptozozin (STZ)-induced diabetic SD rats were randomly assigned to 3 groups: group I/R (n=6) received pancreas transplantation alone (PTA), Group IPC (n=6) received pancreas transplantation alone exposed IPC with 5 minutes ischemic and 5 minutes reperfusion induced by ligating donor's posterior limb three times before ablating donors, Group IPC+DPCPX received same treatment with Group IPC, but separately injected adenosine A1 receptor antagonist 8-cyclopentyl-1, 3-dipropylxanthine (DPCPX) before IPC. The blood glucose, TNF-α in serum, MDA and MPO in pancreatic tissue were monitored before and after reperfusion, and apoptotice cells were stained by TUNEL technique 2 h after reperfusion.ResultsThe blood glucose, TNF-α, apoptotice index (AI), MDA and MPO of group I/R were higher than that of Group IPC and Group IPC+DPCPX after reperfusion(P<0.01), but those of Group IPC+DPCPX were not markedly different with Group IPC(P>0.05). ConclusionIPC in the posterior limbs of rats can protect rat pancreas graft from I/R injury during PTA. Adenosine do not participate in the signal mechanism of this protection.

Key words: pancreas transplantation, ischemic preconditioning, adenosine, rat