《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (08): 618-620.

• 基础研究 • 上一篇    下一篇

缺血预处理对大鼠移植胰细胞凋亡的影响

刘小南1; 霍婷婷2; 王为忠1; 管文贤1; 陈冬利1   

  1. 1.第四军医大学西京医院胃肠外科 陕西西安市 710033;2.第四军医大学西京医院麻醉科 陕西西安市 710033
  • 收稿日期:2005-05-30 出版日期:2005-08-25 发布日期:2005-08-25

Effect of ischemic preconditioning on apoptosis of transplanted pancreas cells in rats

LIUXiao-nan, HUO Ting-ting, WANG Wei-zhong, et al   

  1. The Department of Gastrointestinal Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033,Shanxi, China
  • Received:2005-05-30 Published:2005-08-25 Online:2005-08-25

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摘要： 目的观察缺血预处理对大鼠移植胰细胞凋亡的影响。方法6只正常大鼠为对照组,18只糖尿病SD大鼠随机分为缺血再灌注组(I/R组,n=6)、供胰缺血预处理组(DIPC组,n=6)和受体双后肢缺血预处理组(RIPC组,n=6),均行单纯胰腺移植(18只正常SD大鼠为供体);检测各组大鼠再灌注前、后血糖;再灌注后2h移植胰组织中超氧化物歧化酶(SOD)和髓过氧化物酶(MPO)含量;用TUNEL法观察移植胰组织细胞凋亡情况。结果与I/R组比较,DIPC组和RIPC组大鼠再灌注后的血糖、MPO活性和移植胰组织细胞调亡指数明显降低,移植胰组织中SOD活性明显增高(均为P<0.01)。结论供胰和受体大鼠双后肢缺血预处理能减轻大鼠移植胰细胞的凋亡现象。

关键词: 胰腺移植, 缺血预处理, 缺血再灌注, 细胞凋亡, 大鼠

Abstract: ObjectiveTo observe the effect of ischemic preconditioning (IPC) on apoptosis of transplanted pancreas cells in rats.Methods6 normal SD rats were assigned as control group. 18 steptozozin-induced diabetic SD rats were randomly divided into 3 groups: the I/R group (n= 6, received pancreas transplantation alone), DIPC group (n=6, received pancreas transplantation exposed IPC with 5 min ischemic and 5 min reperfusion twice) and RIPC group (n=6, received pancreas transplantation exposed IPC with 5 minutes ischemic and 5 minutes reperfusion induced by ligating donors' posterior limbs three times before anastomosing vessel). The blood glucose in serum, superoxide dismutase (SOD), myeloperoxidase (MPO), TUNEL cells in graft were monitored.ResultsAfter reperfusion, compared with the I/R group, the mean blood glucose levels, MPO levels and apoptotice index of graft reduced, the mean SOD levels of graft heightened in DIPC and RIPC groups significantly (all P<0.01).ConclusionIschemic preconditioning induced by graft and ligating donors' posterior limbs can reduce apopotosis of transplanted pancreas cells.

Key words: pancreas transplantation, ischemic preconditioning, ischemic reperfusion, apoptosis, rat