《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (09): 693-694.

• 专题 • 上一篇    下一篇

尼莫通对新生大鼠缺氧缺血后学习记忆和海马星形胶质细胞的影响

杨涛1; 谢集建1,2; 常燕群1; 陈运彬1   

  1. 1.广州医学院附属广东省妇女儿童医院儿科 广东广州市 510010;2.武汉大学医学院泰和医院 湖北十堰市 442000
  • 收稿日期:2005-07-07 出版日期:2005-09-25 发布日期:2005-09-25

Effect of nimotop on the long-term learning and memory and astrocytes in perinatal rats after hypoxia-ischemia

YANG Tao, XIE Ji-jian, CHANG Yan-qun, et al   

  1. Department of Pediatrics, Guangdong Maternal and Children Hospital, Guangzhou 510010,Guangdong, China
  • Received:2005-07-07 Published:2005-09-25 Online:2005-09-25

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摘要： 目的研究尼莫通对缺氧缺血性脑损伤(HI)后大鼠学习和记忆能力的影响,并探讨其作用机理。方法7日龄大鼠行右侧颈总动脉接扎后吸入8%氧气2h建立HI模型。随机取13只予尼莫通腹腔注射,每日1次,共5天;至80 d左右行Y迷宫测试,然后用胶质纤维酸性蛋白(GFAP)免疫组化染色标记星形胶质细胞。结果HI组学习记忆能力明显低于正常对照组(P<0.01),尼莫通可提高学习能力,但对记忆无明显作用。HI组海马结构辐射层GFAP阳性细胞数密度高于对照组和治疗组(P<0.05),其他各层差异不显著。结论缺氧缺血性脑损伤可致大鼠学习记忆障碍,可能与缺氧缺血后海马结构中胶质细胞改变导致神经元微环境调节能力减退有关,尼莫通对脑缺氧缺血有一定的改善作用。

关键词: 缺氧缺血, 学习, 记忆, 星形胶质细胞, 尼莫通, 大鼠

Abstract: ObjectiveTo study the effects and mechanisms of hypoxia-ischemia (HI) on long-term learning and memory abilities and astrocytes in hippocampal formation and the efficacy of nimotop in treating hypoxic-ischemic brain damage. MethodsThe rats were subjected to right common carotid artery ligation followed by exposure to 8% oxygen at 37℃ for 2 h and then 13 rat pups received an introperitoneal injection of nimotop per day immediately following cerebral hypoxia-ischemia for 5 days. When the rats were 80-day-old, they were given test of Y-maze to determine their learning and memory abilities, and then their brain tissues were studied by immunohistochemistry for glial fibrillary acidic protein (GFAP) that marked astrocytes. ResultsThe learning and memory abilities of the HI group were lower than those of the normal control and nimotop treated group (P<0.01), nimotop significantly increased Y-maze learning abilities (P<0.05) of rats received HI, but did not affect their memory abilities. The numerical density of GFAP-positive cells in CA1 radiatum stratum of hippocampal formation were markedly higher in the HI group than those in the other two groups (P<0.01), but the others strata showed no difference. ConclusionHypoxic-ischemic brain damage cause rats to disorders of learning and memory that may be correlated with increase astrocyte in hippocampal formation which became easy to be damaged of declining regulation abilities of neurons microenvironment. Nimotop may be effective to counteract hypoxic-ischemic brain damages.

Key words: hypoxia-ischemia, learning, memory, astrocyte, nimotop, rat