《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (09): 716-718.

• 基础研究 • 上一篇    下一篇

电针治疗大鼠慢性脊髓损伤疗效观察及分子机制的实验研究

王新家; 孔抗美; 叶卫莲; 齐伟力; 宋沛松; 蔡燕玲   

  1. 汕头大学医学院第二附属医院脊柱外科 广东汕头市 515041
  • 收稿日期:2005-05-27 出版日期:2005-09-25 发布日期:2005-09-25

Effects of electro-acupuncture on behavioral recovery and its molecular basis in chronic spinal cord injured rats

WANG Xin-jia,KONG Kang-mei, YE Wei-lian,et al   

  1. Department of Spine Surgery, The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China
  • Received:2005-05-27 Published:2005-09-25 Online:2005-09-25

PDF (PC)

被引次数

8

摘要： 目的探索电针治疗慢性脊髓损伤的作用机理。方法采用大鼠后路渐进性脊髓压迫动物模型,然后手术减压,并进行电针治疗。通过体诱发电位和BBB评分观察后肢功能,采用免疫组化和蛋白印迹法观察神经营养因子-3(NT-3)及其受体(TrkC)的变化。结果脊髓损伤后NT-3和TrkC在神经元及胶质细胞表达增强,经过电针治疗后,NT-3和TrkC在神经元和胶质细胞的表达下降。诱发电位检测和BBB评分显示,电针组疗效优于减压组(P<0.05)。结论电针治疗可促进脊髓损伤大鼠的行为功能恢复,这可能是通过内源性神经营养因子及其受体介导的。

关键词: 脊髓损伤, 电针, 神经营养因子-3, 行为, 大鼠

Abstract: ObjectiveTo study the therapeutic mechanisms of electro-acupuncture on chronic spinal cord injury in rats.MethodsA model of chronic spinal cord injury was made. After 60 days, the pressure installs were removed, and the animals were treated with electro-acupuncture. A quantitive analysis was carried out using Basso, Beattie, Bresnahan Locomotor Rating Scale (BBB scale). The expression of Neurotrophin-3 (NT-3) and its receptor TrkC were observed through immunohistochemistrical method and western blotting.ResultsAfter spinal cord injury, enhanced NT-3 and TrkC immunoreactivities were evident in neurons and glial cells. Furthermore, they were diminished in the electro-acupuncture group. Meanwhile, both evoked potentials and BBB scales were improved after EA treatment (P<0.05). ConclusionThe treatment of electro-acupuncture may promote behavioral function recovery of experimental animals, and NT-3 and TrkC may take part in the recovery.

Key words: spinal cord injury, electro-acupuncture, neurotrophin-3, behavior, rat