《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (09): 719-721.

• 基础研究 • 上一篇    下一篇

水通道蛋白4在大鼠创伤性脑水肿中的作用机制

崔向宁; 尹岭; 王玉来   

  1. 1.中日友好医院 北京市 100029;2.解放军总医院神经信息中心 北京市 100853;3.北京中医药大学东方医院 北京市 100078
  • 收稿日期:2005-08-08 出版日期:2005-09-25 发布日期:2005-09-25

Effect of aquaporin 4 on traumatic brain edema

CUI Xiang-ning,YIN Ling,WANG Yu-lai   

  1. China-Jap an Friendship Hospital,Beijing100029, China
  • Received:2005-08-08 Published:2005-09-25 Online:2005-09-25

PDF (PC)

被引次数

54

摘要： 目的探讨大鼠颅脑损伤后脑组织水通道蛋白4(AQP4)的表达规律及其与创伤性脑水肿发生、发展的关系。方法按Feeney自由落体撞击法建立大鼠创伤性脑损伤(TBI)模型,用干/湿重法和Evans蓝(EB)测定法观察大鼠TBI后不同时相脑组织含水量和血脑屏障(BBB)通透性的变化,并采用免疫组织化学方法检测脑组织AQP4蛋白的表达。结果TBI后6h伤侧脑组织含水量及EB含量增高,伤灶周围水肿区星形胶质细胞足突AQP4表达开始增高,24 h达高峰,伤后5 d未恢复正常。AQP4表达与脑组织EB含量的变化呈正相关(r=0.957486,P<0.05)。结论颅脑损伤后,AQP4水平的变化与颅脑损伤后血脑屏障的破坏及脑水肿的形成密切相关。

关键词: 脑创伤, 脑水肿, 水通道蛋白4(AQP4), 血脑屏障, 大鼠

Abstract: ObjectiveTo investigate the dynamic change of cerebral aquaporin 4 (AQP4) expression level during traumatic brain injury (TBI) in rats and the relationship between AQP4 expression and traumatic brain injury and edema. MethodsThe experimental TBI model was established by bumpiness of free falling body according to Feeney's. The changes of brain water content (BWC) and permeability of blood-brain barrier (BBB) were measured by the methods of wet and dry weight and Evans blue fluorometry. Immunohistochemistry was used to determine the change of expression of AQP4 during different periods after TBI. Results6 h after TBI, water content of brain tissue and EB content in the contusion side significantly increased, and the protein expression of AQP4 in contusion marginal region began to increase. They reached summit 24 h after operation and did not come back normal level 5 d after operation. There was significantly positive correlation between protein expression of AQP4 and change of EB content (r=0.957486,P<0.05). ConclusionThe dynamic change of cerebral AQP4 expression level closely related to the destruction of BBB and the formation of cerebral edema during traumatic brain injury.

Key words: cerebral trauma, cerebral edema, aquaporin 4(AQP4), blood-brain barrier, rat