《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (10): 785-786.

• 专题 • 上一篇    下一篇

低密度、氧化低密度脂蛋白对内皮细胞分泌内皮素的影响

张敏1; 胥学伟2; 李小鹰3   

  1. 1.解放军205医院神经消化科 辽宁锦州市 121000;2.解放军205医院内四科 辽宁锦州市 121000;3.解放军总医院南六科 北京市 100853
  • 收稿日期:2005-04-18 出版日期:2005-10-25 发布日期:2005-10-25

Effect of low density lipoprotein and oxidized low density lipoprotein on the secretion of endothelin in endothelial cells

ZHANG Min,XUXue-wei, LI Xiao-ying   

  1. The Department of Geriatric Cardiology, No.205Hospital of PLA, Jinzhou 121000, Liaoning,China
  • Received:2005-04-18 Published:2005-10-25 Online:2005-10-25

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被引次数

3

摘要： 目的观察低密度脂蛋白(LDL)、氧化低密度脂蛋白(ox-LDL)对内皮细胞分泌内皮素(ET)的影响,以及后者在体外是否对前者具有氧化修饰作用。方法采用不同浓度的LDL、ox-LDL及LDL+ox-LDL与脐静脉内皮细胞株ECV-304进行孵育,24 h后分别收集细胞和上清液,采用放免分析法测定ET含量。结果LDL、ox-LDL均能促进内皮细胞合成、分泌ET,但ox-LDL的作用更显著,而LDL+ox-LDL组上清ET浓度大于两者单独作用之和。结论LDL、ox-LDL均能促进内皮细胞合成、分泌ET,但后者的作用更为明显,且在体外对LDL具有氧化修饰作用。

关键词: 脂蛋白, 氧化低密度脂蛋白, 内皮细胞, 内皮素

Abstract: ObjectiveTo investigate the effect of low density lipoprotein(LDL) and oxidized low density lipoprotein(ox-LDL) on the secretion of endothelin(ET) and whether LDL to be oxidized and modified by ox-LDL in vitro.MethodsEndothelial cell strain ECV-304 was incubated with different concentration of LDL(50 and 100 mg/L),ox-LDL(50 and 100 mg/L) or LDL+ox-LDL(50mg/L) for 24 hours,then cells and cultured medium were collected.ET in cells and cultured medium were detected with radioimmunoassay.ResultsBoth of LDL and ox-LDL enhanced the secretion of ET in cultured endothelial cells;the effect of ox-LDL was more markedly than LDL.LDL+ox-LDL had a greater effect than any of the two lipoproteins alone.ConclusionLDL and ox-LDL may have the ability to promote the secretion of ET in cultured endothelial cells and LDL can be oxidized and modified by ox-LDL in vitro.

Key words: low density lipoprotein, oxidized low density lipoprotein, endothelial cell, endothelin