《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (10): 808-810.

• 基础研究 • 上一篇    下一篇

血管内皮生长因子和血小板凝集素-1在膀胱癌中的表达

张冰; 黎玮; 蔡文清; 李刚   

  1. 1.石家庄市中心医院泌尿外科 河北石家庄市 050011;2.河北医科大学第二医院泌尿外科 河北石家庄市 050011
  • 收稿日期:2004-12-13 出版日期:2005-10-25 发布日期:2005-10-25

Expression of vascular endothlial growth factor and throbospondin-1 in bladder cancer

ZHANG Bing, LI Wei, CAI Wen-qing, et al   

  1. The Department of Urology, The Shijiazhuang Center Hospital, Shijiazhuang 050011, Hebei, China
  • Received:2004-12-13 Published:2005-10-25 Online:2005-10-25

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被引次数

1

摘要： 目的观察血管内皮生长因子(VEGF)与血小板凝集素-1(TSP-1)在不同分级的膀胱肿瘤中的表达。方法2000年4月～2003年9月的70例膀胱肿瘤患者切除标本,其中G1期28例、G2期27例、G3期15例(采用WHO病理分级),采用免疫组化法对标本进行染色,VEGF和TSP-1的表达均采用平均血管密度(MVD)表示,SPSS 10.0软件进行统计学分析,然后采用免疫荧光双染判断共表达,用激光扫描共聚焦显微镜观察。结果VEGF表达的MVD值与肿瘤分级呈正相关;G1与G2期有非常显著性差异(P<0.01),G2期与G3无显著性差异(P>0.1);TSP-1表达的MVD值随着肿瘤分级增加而降低,但无相关关系,但G1期与G2、G3期有非常显著性差异(P<0.01),G2期与G3无显著性差异(P>0.1);在G1期,TSP-1的表达与VEGF的表达无相关关系(rs=0.167,P>0.1);激光扫描荧光双染显示,VEGF与TSP-1可以同时表达。结论VEGF的表达随膀胱肿瘤分级增加而增强;VEGF是促进肿瘤形成的因素;TSP-1是抑制肿瘤血管形成的因素,但其抑制作用仅发生在初期;荧光双染仅可提供共同表达的证据,不能判断表达的高低。

关键词: 膀胱癌, 血管内皮生长因子(VEGF), 血小板凝集素-1(TSP-1), 免疫组化法, 平均血管密度, 免疫荧光双染, 激光扫描

Abstract: ObjectiveTo observe the expression of vascular endothlial growth factor(VEGF) and throbospondin-1(TSP-1) in different grade bladder cancer.MethodsSpecimens of 70 cases of bladder transitional cell cancer including Grade Ⅰ 28 cases,Grade Ⅱ 27 cases,Grade Ⅲ 15 cases(according to WHO pathological grade) were stained with immunohistochemistry method.The expressing values of VEGF and TSP-1 were calculated by mean vascular density(MVD).All data was analyzed with SPSS 10.0,and with expression of VEGF and TSP-1 was detected with immunohistochemisrty double staining.Finally,the result of expression was observed by laser scan with focus microscope.ResultsThere was a positive correlation between the cancer grade and the expression of VEGF,and the expression of Grade Ⅰ was significant difference with that of Grade Ⅱ(P<0.01),but there was no difference between Grade Ⅱ and Grade Ⅲ(P>0.1).Expression of TSP-1 decreased with grade increasing but not showing a negative correlation,while,Grade Ⅰ was significant difference with that of Grade Ⅱ and Grade Ⅲ(P<0.01),Grade Ⅱ was not differcnt with Grade Ⅲ(P>0.1).In 28 Grade Ⅰ cases,values of MVD of VEGF and TSP-1 had no correlation(rs)=0.167,P>0.1).Double immunostaining detecting with laser scan with focus microcope indicate showed that VEGF and TSP-1 appeared in same bladder cancer specimen.ConclusionExpression of VEGF is gradually increased from Grade Ⅰ to Grade Ⅲ,and VEGF is a promoting factor of angiogenesic bladder cancer.Expression of TSP-1 is the strongest in Grade Ⅰ cases,and TSP-1 is an inhibiting angiogenesic factor,which inhibiting function only in early stage.Immunofluorescent staining can only provide evidence of together expression of VEGF and TSP-1,cannot evaluate the degree of expression.

Key words: bladder cancer, vascular endothlial growth factor(VEGF), thrombospondin-1(TSP-1), immunohistochemistry, mean vascular density, immunofluorescent double staining, laser scan focus microscope