《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (10): 816-819.

• 基础研究 • 上一篇    下一篇

骨软骨镶嵌移植修复关节软骨缺损的实验研究

东海潮; 李靖年; 南丰   

  1. 大连医科大学附属第二医院骨外科 辽宁大连市 116027
  • 收稿日期:2005-07-05 出版日期:2005-10-25 发布日期:2005-10-25

Experimental study of bone and cartilage transplantation to repair the defect of articular cartilage

DONG Hai-chao, LI Jing-nian,NAN-feng   

  1. The Department of Orthopaedics, The Second Affiliated Hospital of Dalian Medical University, Dalian 116027,Liaoning, China
  • Received:2005-07-05 Published:2005-10-25 Online:2005-10-25

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被引次数

8

摘要： 目的探讨自体和异体骨软骨移植的效果和可行性。方法将63只健康成年家兔同侧膝关节造成实验性缺损,随机将动物分成A组(自体移植组,27只)、B组(异体移植组,27只)和C组(对照组,9只)。A组再分为2柱骨软骨移植不限制运动组(A2M组)、限制运动组(A2N组)和4柱骨软骨移植不限制运动组(A4M组);B组同样再分为B2M组、B2N组和B4M组,每组9只动物,分别做2柱、4柱自体和异体非负重区骨软骨柱移植。对实验动物进行大体观察、测量关节活动度及修复移植组织厚度、组织学光镜和电镜检查。结果A组软骨面愈合修复满意,其中A4M组更佳,光镜、电镜检查示移植物与骨床骨性愈合,陷落极少,无明显退变,成熟软骨细胞更多,关节活动度和修复组织与周边正常软骨平均厚度的百分比均优于A2M组(P≤0.05)。B组修复软骨缺损效果不佳。结论小直径、多数目的骨软骨柱更易重塑较大面积的软骨缺损轮廓;新鲜无处理的同种异体骨软骨镶嵌移植修复家兔关节软骨缺损排斥反应及吸收破坏严重。

关键词: 膝关节, 软骨缺损, 移植

Abstract: ObjectiveTo explore the effect and feasibility of autograft and allograft bone and cartilage transplantation.Methods63 rabbits were made experimental defect in the same side knee joint,and were divided randomly into group A(autograft group),group B(allograft group) and group C(control group).Group A was divided further into 2 column limiting motion subgroup(A2M group),not limiting motion subgroup(A2N group) and 4 column subgroup(A4M group).In the same way,group B was divided further into B2M group,B2N group and B4M group.Every subgroup included nine rabbits,and the transplantation was made in no weight-bear area of knee joint.The generally observation were made,the joint movable range and the thickness of the repairing tissue were measured,histology change of transplanted tissue(through light,electronic microscope)were observed.ResultsGroup A had a satisfactory result,and the result of no limiting groups were better.The result of A4M group was better than A2M group (P≤0.05).The result of group B was not good.ConclusionThe bone-cartilage column of small diameter and general number are beneficial in repairing the defect of joint cartilage.The allograft bone and cartilage transplantation can cause seriously immunoreaction and the absorption of cartilage pole,and is detrimental to repair the defect of joint cartilage.

Key words: knee joint, cartilage defect, transplantation