《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (11): 922-923.

• 基础研究 • 上一篇    下一篇

原代培养人嗜铬细胞瘤细胞的生长和分泌特征

郑欣1; 曾正陪1; 李明1; 严维刚2; 李汉忠2   

  1. 1.中国医学科学院,中国协和医科大学,北京协和医院内分泌科 北京市 100730;2.中国医学科学院,中国协和医科大学,北京协和医院泌尿外科 北京市 100730
  • 收稿日期:2005-07-25 出版日期:2005-11-25 发布日期:2005-11-25

Characteristics of growth and secretion in primarily cultured human pheochromocytoma cells

ZHENG Xin, ZENG Zheng-pei, LI Ming, et al   

  1. Department of Endocrinology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2005-07-25 Published:2005-11-25 Online:2005-11-25

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摘要： 目的观察体外培养的人嗜铬细胞瘤细胞生长和分泌的特征。方法连续分次消化人嗜铬细胞瘤组织,进行细胞原代培养;用细胞计数方法测定生长曲线,同时收集细胞液,用高效液相色谱分析法测定其中儿茶酚胺的水平,绘制时间-分泌曲线。结果在培养的第3天,原代培养的人嗜铬细胞瘤细胞数目开始逐渐增多,第6天达高峰,第7天后细胞数目减少;在细胞培养第1～2天,细胞液中去甲肾上腺素(NE)、肾上腺素(E)浓度明显下降,第2～8天无明显变化,多巴胺(DA)浓度在1～8天无明显变化。结论人嗜铬细胞瘤在体外培养时,增殖不显著,但分化明显,其儿茶酚胺的水平,在培养最初明显下降。

关键词: 嗜铬细胞瘤, 细胞培养, 儿茶酚胺

Abstract: ObjectiveTo investigate the characteristics of growth and secretion in primarily cultured human pheochromocytoma cells.MethodsThe human pheochromocytoma tissues were digested successively,and the tumor cells were cultured.The growth curves of three tumors were plotted by cell count.At the same time,the catecholomine in the medium was measured with high performance liquid chromatography.ResultsThe number of primarily cultured human pheochromocytoma cells increased gradually from first to sixth day after plating,and then the cell number began to decrease from seventh day.The levels of norepinephrine(NE) and epinephrine(E) in the medium of cultured cells significantly decreased on first and second day,and the levels of NE and E had no significant difference on the second to eighth day.The levels of dopamine in the medium had no significant difference on the first to eighth day.ConclusionThe primarily cultured pheochromocytoma cells differentiate obviously after plating,but have no significant proliferation.The levels of catecholomine in the medium are decreased significantly on the first and second day.

Key words: pheochromocytoma, cell culture, catecholomine