《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (11): 927-928.

• 基础研究 • 上一篇    下一篇

Ghrelin对GH3细胞膜电位的作用

韩雪峰; 朱运龙; 陈晨   

  1. 第四军医大学生理学教研室 陕西西安市 710032
  • 收稿日期:2005-05-26 出版日期:2005-11-25 发布日期:2005-11-25

Reduction of membrane potential in GH3 cells with ghrelin

HAN Xue-feng, ZHU Yun-long, CHEN Chen   

  1. Department of Physiology, the Fourth Military Medical University,710032 Xi'An, Shanxi, China
  • Received:2005-05-26 Published:2005-11-25 Online:2005-11-25

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摘要： 目的观察ghrelin对GH3细胞膜电位的作用。方法用Nystatin打孔全细胞记录跨膜电流,观察ghrelin组和对照组GH3细胞膜电位。结果对照组细胞的膜电位为-(72±4.78)mV,在ghrelin处理后,膜电位减小到(54.8±4.58)mV。结论ghrlein可以快速、可逆、显著性地降低GH3细胞膜电位。

关键词: ghrelin, 膜片钳, 膜电位, 生长激素

Abstract: ObjectiveTo investigate the in vitro effect of ghrelin on membrane membrane potentail of GH3 cells.MethodsNystatin-perforated whole-cell recording configuration was used.ResultsThe membrane potentail of GH3 was decreased from-(72±4.78) mV to(54.8±4.58) mV(P<0.01) after application of ghrelin(10-7～10-8 M)ConclusionGhrelin can transiently and significantly decreased the amplitude of membrane potentail,which may contribute to the ghrelin-stimulated GH secretion from GH3 cells.

Key words: ghrelin, patch-clamp, membrane potentail, growth hormone