《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (12): 1005-1006.

• 基础研究 • 上一篇    下一篇

直流小电场诱导乳腺癌细胞p21、p16基因表达与凋亡的初步研究

李晓瑛1; 王兴林1; 赵坡2   

  1. 1.解放军总医院理疗科 北京市 100853;2.解放军总医院病理科 北京市 100853
  • 收稿日期:2005-04-29 出版日期:2005-12-25 发布日期:2005-12-25

Apoptosis of breast cancer cell line induced by small direct-current electric field and its related mechanism in expression of p21 and p16 genes

LI Xiao-ying, WANG Xing-lin, ZHAO Po   

  1. The Department of Physiotherapy, The General Hospital of PLA, Beijing 100853, China
  • Received:2005-04-29 Published:2005-12-25 Online:2005-12-25

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摘要： 目的观察直流小电场对乳腺癌细胞P21、P16基因mRNA表达的诱导作用及与凋亡的关系。方法应用体外直流小电场作用于乳腺癌细胞系MCF7,观察其对乳腺癌细胞重要基因P21、P16表达以及凋亡的影响。结果直流小电场对乳腺癌细胞有明显的凋亡诱导作用,200mV/mm组癌细胞大量脱落;电场组凋亡肿瘤细胞数随电场强度升高而增多(均为P<0.01),P21、P16基因mRNA表达明显升高。结论直流小电场对乳腺癌细胞具有明显的凋亡诱导作用,可能与P21、P16基因表达相关。

关键词: 乳腺肿瘤, 直流电场, 基因

Abstract: ObjectiveTo observe the apoptosis of breast cancer cells induced by small direct-current electric field (EF) and its related possible mechanism in expression of p21 and p16 genes.MethodsSmall direct-current EF was applied in human breast cancer cell line MCF7 in vitro and the effect of EF on apoptosis and expression of p21 and p16 important genes of breast cancer cells was observed.ResultsThe small direct-current EF had an obvious apoptosis inducing effect on breast cancer cells. A lot of cancer cells were sloughed from culture dish by 200 mV/mm of the EF strength. The number of the apoptotic cancer cells was gradually significantly increased and mRNA expressions of p21 and p16 genes were raised following the EF strength being up (P<0.01).ConclusionThe small direct-current EF has an obvious apoptosis inducing effect on breast cancer cells. It may be related with the expression of p21 and p16 genes.

Key words: breast neoplasm, direct-current electric field, gene