《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (12): 965-967.

• 专题 •    下一篇

人类羊膜细胞表达神经干细胞特异性蛋白研究

蔡哲1; 潘琳1; 舒峻1; 张岚1; 郭艳茹1; 耿同超2; 牟亮3; 左萍萍3   

  1. 1.中日友好医院临床医学研究所免疫学研究室 北京市 100029;2.清华大学附属玉泉医院神经内科 北京市 100049;3.中国医学科学院基础医学研究所药理学研究室 北京市 100005
  • 收稿日期:2005-10-27 出版日期:2005-12-25 发布日期:2005-12-25

Specific proteins of neural stem cell expressed by human amnion cells

CAI Zhe, PAN Lin, SHU Jun, et al   

  1. The Department of Immunology, Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China
  • Received:2005-10-27 Published:2005-12-25 Online:2005-12-25

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被引次数

25

摘要： 目的利用神经干细胞特异性标志蛋白鉴定人类羊膜组织中多分化潜能神经前体细胞的存在。方法利用免疫组织化学法检测人类羊膜组织和培养人类羊膜细胞中巢蛋白(nestin)、胶质原纤维酸性蛋白(GFAP)、musashi-1、波形蛋白(vimentin)和PSA-NCAM等神经干细胞特异性标志蛋白的表达。结果人羊膜组织中存在nestin/GFAP双阳性细胞,此外,还表达musashi-1、vimentin和PSA-NCAM等神经干细胞特异性标志蛋白;培养羊膜细胞中存在vimentin和PSA-NCAM阳性细胞,以及nestin/GFAP双阳性细胞。结论羊膜组织和培养羊膜细胞中有神经干细胞特异性标记蛋白的表达。

关键词: 人类羊膜细胞, 神经前体细胞, 巢蛋白, musashi-1

Abstract: ObjectiveTo characterize the neural progenitor cell in the human amnion mesenchyme and epithelial layer with specific mark proteins of neural stem cell.MethodsExpressions of specific mark proteins of neural stem cell including nestin, glial fibrillary acidic protein (GFAP), musashi-1, vimentin and PSA-NCAM in human amnion tissue and cultured amniotic cells were determined by immunohistochemistry and immunofluorescence staining.ResultsExpressions of pluripotent neural stem cell specific makers (nestin, musashi-1, vimentin and PSA-NCAM) were detected in the human amnion mesenchyme and epithelial layer. In addition, cultured amniotic cells were expressed several neural stem cell specific markers including nestin, GFAP and PSA-NCAM. Nestin+ and GFAP+ double positive cells were identified in the human amnion tissue and cultured amniotic cells by immunohistochemistry and immunofluorescence staining.ConclusionSpecific mark proteins of neural stem cell are expressed in human amnion tissue and cultured amniotic cells.

Key words: human amnion cell, neural progenitor cell, nestin, musashi-1