《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (12): 968-970.

• 专题 • 上一篇    下一篇

胚胎脊髓移植联合应用bFGF对脊髓神经组织损伤的早期保护作用

郭风劲; 李新志; 许涛; 陈安民   

  1. 华中科技大学同济医学院附属同济医院骨科 湖北武汉市 430030
  • 收稿日期:2005-07-01 出版日期:2005-12-25 发布日期:2005-12-25

Early protective effects of transplantation of fetal spinal cord and bFGF on spinal cord injury in adult rats

GUO Feng-jin, LI Xin-zhi, XU Tao, et al   

  1. The Department of Orthopaedics, Tongji Hospital, Tongji Medical College, Huazhong University of Sci- ence and Technology, Wuhan 430030, Hubei, China
  • Received:2005-07-01 Published:2005-12-25 Online:2005-12-25

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2

摘要： 目的探讨脊髓损伤后,联合应用胚胎脊髓(FSC)移植和外源性碱性成纤维细胞生长因子(bFGF)对损伤脊髓组织早期Ca2+、Mg2+的影响。方法利用Wistar大鼠建立脊髓损伤模型;造模后,A组经蛛网膜下腔导管注入5ml FSC细胞悬液;B组注入20μl bFGF与FSC细胞悬液;C组(对照组)不做处理。术后6h、24h取A组、B组和C组动物损伤区脊髓组织测定水和Ca2+、Mg2+含量;术后5d取损伤脊髓组织电镜下观察结构变化。结果C组损伤区脊髓组织水含量增多,Ca2+水平升高,Mg2+水平下降,白质内髓鞘结构紊乱,囊性变严重;A组和B组上述变化均较C组改善。结论脊髓损伤后应用FSC移植和bFGF可减轻离子失衡,从而对继发性损害有抑制作用。

关键词: 胚胎脊髓细胞, 碱性成纤维细胞生长因子, 脊髓损伤

Abstract: ObjectiveTo explore the early effects of transplantation of fetal spinal cord (FSC) and basic fibroblast growth factor (bFGF) on calcium, magnesium of injured spinal cord.MethodsThe spinal cord injury (SCI) animal model was established with Wistar rats. The rats were divided into 3 groups. The group A was received FSC cells suspension 5 ml after injury. The group B was received FSC cells suspension and bFGF 20 μl. The group C was not treated with any therapy. The values of Ca2+, Mg2+ of injured spinal cord tissues were measured at 6, 24 hours after injury. The morphyological results were observed by electron microscope on the fifth day.ResultsThe degree of ionic disorder in FSC+bFGF treated rats was strikingly ameliorated and the contents of H2O also decreased. The morphyological finding revealed that in FSC+bFGF treated rats, the damage of gray and white matters was slight in contrast to those of control group rats, which appeared a typically damage.ConclusionTransplantation of FSC and bFGF has some protective effects on contused spinal cord in rats.

Key words: fetal spinal cord cell, basic fibroblast growth factor, spinal cord injury